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Physiological Roles of Bradykinin and Involvement of Bradykinin B2 Receptor in Urethral Function in Humans and Animals.
Fujimoto, Kenichiro; Yoshino, Taiji; Nakajima, Satoko; Yuyama, Hironori; Masuda, Noriyuki; Takeda, Masahiro.
Afiliación
  • Fujimoto K; Drug Discovery Research, Astellas Pharma Inc., Tsukuba, Japan.
  • Yoshino T; Drug Discovery Research, Astellas Pharma Inc., Tsukuba, Japan.
  • Nakajima S; Drug Discovery Research, Astellas Pharma Inc., Tsukuba, Japan.
  • Yuyama H; Drug Discovery Research, Astellas Pharma Inc., Tsukuba, Japan.
  • Masuda N; Drug Discovery Research, Astellas Pharma Inc., Tsukuba, Japan.
  • Takeda M; Drug Discovery Research, Astellas Pharma Inc., Tsukuba, Japan.
Low Urin Tract Symptoms ; 9(3): 187-191, 2017 Sep.
Article en En | MEDLINE | ID: mdl-27167682
OBJECTIVE: We investigated the role of bradykinin in urethral function by examining contractile responses in urethral smooth muscle strips isolated from humans and the intraurethral pressure in rats and dogs. METHODS: The contractile responses of human urethral tissue for bradykinin (0.01-10 µmol/L) were examined, and changes in intraurethral pressure induced by bradykinin (0.003-10 µg/kg) in anesthetized rats or dogs were measured. In addition, the effects of pretreatment with the bradykinin B2 receptor antagonist FK3657 were also examined. RESULTS: In smooth muscle strips obtained from human urethra, bradykinin induced contraction, which was inhibited by FK3657 in a concentration-dependent manner. In anesthetized rats and dogs, intravenously administered bradykinin dose-dependently increased intraurethral pressure. FK3657 shifted the intraurethral pressure dose-response curve for bradykinin to the right in rats. The bradykinin-induced elevation of intraurethral pressure was also dose-dependently inhibited by FK3657 in dogs. CONCLUSIONS: The present study provides evidence that bradykinin elicits urethral smooth muscle contraction via the bradykinin B2 receptor, suggesting the potential utility of this receptor as a novel target for the treatment of voiding dysfunction.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Uretra / Bradiquinina / Receptor de Bradiquinina B2 Límite: Animals Idioma: En Revista: Low Urin Tract Symptoms Año: 2017 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Australia

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Uretra / Bradiquinina / Receptor de Bradiquinina B2 Límite: Animals Idioma: En Revista: Low Urin Tract Symptoms Año: 2017 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Australia