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Plant microRNAs as novel immunomodulatory agents.
Cavalieri, Duccio; Rizzetto, Lisa; Tocci, Noemi; Rivero, Damariz; Asquini, Elisa; Si-Ammour, Azeddine; Bonechi, Elena; Ballerini, Clara; Viola, Roberto.
Afiliación
  • Cavalieri D; Research and Innovation Centre, Fondazione Edmund Mach, via E. Mach 1, 38010 San Michele all'Adige (TN), Italy.
  • Rizzetto L; Department of Biology, University of Florence, Via Madonna del Piano 6, 50019 Sesto Fiorentino (FI), Italy.
  • Tocci N; Research and Innovation Centre, Fondazione Edmund Mach, via E. Mach 1, 38010 San Michele all'Adige (TN), Italy.
  • Rivero D; Research and Innovation Centre, Fondazione Edmund Mach, via E. Mach 1, 38010 San Michele all'Adige (TN), Italy.
  • Asquini E; Dipartimento di Neuroscienze, Psicologia, Area del Farmaco e Salute del Bambino (Neurofarba), University of Florence, viale Pieraccini 6, 50139 Firenze, Italy.
  • Si-Ammour A; Research and Innovation Centre, Fondazione Edmund Mach, via E. Mach 1, 38010 San Michele all'Adige (TN), Italy.
  • Bonechi E; Research and Innovation Centre, Fondazione Edmund Mach, via E. Mach 1, 38010 San Michele all'Adige (TN), Italy.
  • Ballerini C; Dipartimento di Neuroscienze, Psicologia, Area del Farmaco e Salute del Bambino (Neurofarba), University of Florence, viale Pieraccini 6, 50139 Firenze, Italy.
  • Viola R; Dipartimento di Neuroscienze, Psicologia, Area del Farmaco e Salute del Bambino (Neurofarba), University of Florence, viale Pieraccini 6, 50139 Firenze, Italy.
Sci Rep ; 6: 25761, 2016 05 11.
Article en En | MEDLINE | ID: mdl-27167363
An increasing body of literature is addressing the immuno-modulating functions of miRNAs which include paracrine signaling via exosome-mediated intercellular miRNA. In view of the recent evidence of intake and bioavailability of dietary miRNAs in humans and animals we explored the immuno-modulating capacity of plant derived miRNAs. Here we show that transfection of synthetic miRNAs or native miRNA-enriched fractions obtained from a wide range of plant species and organs modifies dendritic cells ability to respond to inflammatory agents by limiting T cell proliferation and consequently dampening inflammation. This immuno-modulatory effect appears associated with binding of plant miRNA on TLR3 with ensuing impairment of TRIF signaling. Similarly, in vivo, plant small RNAs reduce the onset of severity of Experimental Autoimmune Encephalomyelities by limiting dendritic cell migration and dampening Th1 and Th17 responses in a Treg-independent manner. Our results indicate a potential for therapeutic use of plant miRNAs in the prevention of chronic-inflammation related diseases.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN de Planta / Fragaria / MicroARNs / Factores Inmunológicos Límite: Animals / Female / Humans Idioma: En Revista: Sci Rep Año: 2016 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN de Planta / Fragaria / MicroARNs / Factores Inmunológicos Límite: Animals / Female / Humans Idioma: En Revista: Sci Rep Año: 2016 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido