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Isolation and Characterization of Dromedary Camel Coronavirus UAE-HKU23 from Dromedaries of the Middle East: Minimal Serological Cross-Reactivity between MERS Coronavirus and Dromedary Camel Coronavirus UAE-HKU23.
Woo, Patrick C Y; Lau, Susanna K P; Fan, Rachel Y Y; Lau, Candy C Y; Wong, Emily Y M; Joseph, Sunitha; Tsang, Alan K L; Wernery, Renate; Yip, Cyril C Y; Tsang, Chi-Ching; Wernery, Ulrich; Yuen, Kwok-Yung.
Afiliación
  • Woo PC; State Key Laboratory of Emerging Infectious Diseases, the University of Hong Kong, Pokfulam, Hong Kong. pcywoo@hku.hk.
  • Lau SK; Department of Microbiology, the University of Hong Kong, Pokfulam, Hong Kong. pcywoo@hku.hk.
  • Fan RY; Research Centre of Infection and Immunology, the University of Hong Kong, Pokfulam, Hong Kong. pcywoo@hku.hk.
  • Lau CC; Carol Yu Centre for Infection, the University of Hong Kong, Pokfulam, Hong Kong. pcywoo@hku.hk.
  • Wong EY; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou 310006, China. pcywoo@hku.hk.
  • Joseph S; State Key Laboratory of Emerging Infectious Diseases, the University of Hong Kong, Pokfulam, Hong Kong. skplau@hku.hk.
  • Tsang AK; Department of Microbiology, the University of Hong Kong, Pokfulam, Hong Kong. skplau@hku.hk.
  • Wernery R; Research Centre of Infection and Immunology, the University of Hong Kong, Pokfulam, Hong Kong. skplau@hku.hk.
  • Yip CC; Carol Yu Centre for Infection, the University of Hong Kong, Pokfulam, Hong Kong. skplau@hku.hk.
  • Tsang CC; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou 310006, China. skplau@hku.hk.
  • Wernery U; Department of Microbiology, the University of Hong Kong, Pokfulam, Hong Kong. rachelfyy2004@yahoo.com.hk.
  • Yuen KY; Department of Microbiology, the University of Hong Kong, Pokfulam, Hong Kong. candylaucy@gmail.com.
Int J Mol Sci ; 17(5)2016 May 07.
Article en En | MEDLINE | ID: mdl-27164099
Recently, we reported the discovery of a dromedary camel coronavirus UAE-HKU23 (DcCoV UAE-HKU23) from dromedaries in the Middle East. In this study, DcCoV UAE-HKU23 was successfully isolated in two of the 14 dromedary fecal samples using HRT-18G cells, with cytopathic effects observed five days after inoculation. Northern blot analysis revealed at least seven distinct RNA species, corresponding to predicted subgenomic mRNAs and confirming the core sequence of transcription regulatory sequence motifs as 5'-UCUAAAC-3' as we predicted previously. Antibodies against DcCoV UAE-HKU23 were detected in 58 (98.3%) and 59 (100%) of the 59 dromedary sera by immunofluorescence and neutralization antibody tests, respectively. There was significant correlation between the antibody titers determined by immunofluorescence and neutralization assays (Pearson coefficient = 0.525, p < 0.0001). Immunization of mice using recombinant N proteins of DcCoV UAE-HKU23 and Middle East respiratory syndrome coronavirus (MERS-CoV), respectively, and heat-inactivated DcCoV UAE-HKU23 showed minimal cross-antigenicity between DcCoV UAE-HKU23 and MERS-CoV by Western blot and neutralization antibody assays. Codon usage and genetic distance analysis of RdRp, S and N genes showed that the 14 strains of DcCoV UAE-HKU23 formed a distinct cluster, separated from those of other closely related members of Betacoronavirus 1, including alpaca CoV, confirming that DcCoV UAE-HKU23 is a novel member of Betacoronavirus 1.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Camelus / Reacciones Cruzadas / Coronavirus del Síndrome Respiratorio de Oriente Medio Límite: Animals / Humans Idioma: En Revista: Int J Mol Sci Año: 2016 Tipo del documento: Article País de afiliación: Hong Kong Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Camelus / Reacciones Cruzadas / Coronavirus del Síndrome Respiratorio de Oriente Medio Límite: Animals / Humans Idioma: En Revista: Int J Mol Sci Año: 2016 Tipo del documento: Article País de afiliación: Hong Kong Pais de publicación: Suiza