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E-Prostanoid 2 Receptor Overexpression Promotes Mesenchymal Stem Cell Attenuated Lung Injury.
Han, Jibin; Lu, Xiaomin; Zou, Lijuan; Xu, Xiuping; Qiu, Haibo.
Afiliación
  • Han J; Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast University , Nanjing, China .
  • Lu X; Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast University , Nanjing, China .
  • Zou L; Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast University , Nanjing, China .
  • Xu X; Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast University , Nanjing, China .
  • Qiu H; Department of Critical Care Medicine, Zhongda Hospital, School of Medicine, Southeast University , Nanjing, China .
Hum Gene Ther ; 27(8): 621-30, 2016 08.
Article en En | MEDLINE | ID: mdl-27158855
Mesenchymal stem cells (MSCs) represent a promising approach for the treatment of acute respiratory distress syndrome (ARDS). However, their low efficiency in homing to injured lung tissue limits their therapeutic effect. Prostaglandin E2 (PGE2) biosynthesis substantially enhances the inflammatory response of the tissue. Moreover, it also facilitates the migration of MSCs by activating the E-prostanoid 2 (EP2) receptor in vitro. Given these observations, it would seem reasonable that PGE2 might act as a chemokine to promote the migration of MSCs through activation of the EP2 receptor. Herein, we confirmed that PGE2 was significantly increased in lung tissue as a result of stimulation by LPS. In addition, we constructed a lentiviral vector carrying the EP2 gene, which was successfully transduced into MSCs (MSCs-EP2). Near-infrared imaging and immunofluorescence showed that compared with MSCs-GFP, MSCs-EP2 significantly enhanced MSC homing to injured lung tissue. Moreover, the diminished amounts of Evans blue in homogeneous lung parenchyma in vivo indicated, in comparison with MSCs-GFP, that MSCs-EP2 significantly decreased LPS-induced pulmonary vascular permeability. In addition, administration of MSCs-EP2 largely decreased the levels of interleukin-1ß and tumor necrosis factor-α compared with that observed after administration of MSCs-GFP at both 24 and 72 hr. Our results suggested that treatment with MSCs-EP2 markedly enhanced MSC homing to damaged lung tissue and, in addition, improved both lung inflammation and permeability. Thus, MSCs and EP2 combination gene therapy could markedly facilitate MSC homing to areas of inflammation, representing a novel strategy for MSC-based gene therapy in inflammatory diseases.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Terapia Genética / Trasplante de Células Madre Mesenquimatosas / Lesión Pulmonar / Subtipo EP2 de Receptores de Prostaglandina E / Células Madre Mesenquimatosas Tipo de estudio: Etiology_studies Límite: Animals Idioma: En Revista: Hum Gene Ther Asunto de la revista: GENETICA MEDICA / TERAPEUTICA Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Terapia Genética / Trasplante de Células Madre Mesenquimatosas / Lesión Pulmonar / Subtipo EP2 de Receptores de Prostaglandina E / Células Madre Mesenquimatosas Tipo de estudio: Etiology_studies Límite: Animals Idioma: En Revista: Hum Gene Ther Asunto de la revista: GENETICA MEDICA / TERAPEUTICA Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos