Your browser doesn't support javascript.
loading
Nutrient-induced glucagon like peptide-1 release is modulated by serotonin.
Ripken, Dina; van der Wielen, Nikkie; Wortelboer, Heleen M; Meijerink, Jocelijn; Witkamp, Renger F; Hendriks, Henk F J.
Afiliación
  • Ripken D; Top Institute Food and Nutrition, Nieuwe Kanaal 9A, Wageningen, 6709 PA, The Netherlands; Netherlands Organization for Applied Scientific Research TNO, Utrechtseweg 48, 3704 HE, Zeist, The Netherlands; Division of Human Nutrition, Wageningen University, Bomenweg 2, 6703 HD, Wageningen, The Netherlan
  • van der Wielen N; Top Institute Food and Nutrition, Nieuwe Kanaal 9A, Wageningen, 6709 PA, The Netherlands; Division of Human Nutrition, Wageningen University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands.
  • Wortelboer HM; Netherlands Organization for Applied Scientific Research TNO, Utrechtseweg 48, 3704 HE, Zeist, The Netherlands.
  • Meijerink J; Division of Human Nutrition, Wageningen University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands.
  • Witkamp RF; Division of Human Nutrition, Wageningen University, Bomenweg 2, 6703 HD, Wageningen, The Netherlands.
  • Hendriks HF; Top Institute Food and Nutrition, Nieuwe Kanaal 9A, Wageningen, 6709 PA, The Netherlands.
J Nutr Biochem ; 32: 142-50, 2016 06.
Article en En | MEDLINE | ID: mdl-27142747
Glucagon like peptide-1 (GLP-1) and serotonin are both involved in food intake regulation. GLP-1 release is stimulated upon nutrient interaction with G-protein coupled receptors by enteroendocrine cells (EEC), whereas serotonin is released from enterochromaffin cells (ECC). The central hypothesis for the current study was that nutrient-induced GLP-1 release from EECs is modulated by serotonin through a process involving serotonin receptor interaction. This was studied by assessing the effects of serotonin reuptake inhibition by fluoxetine on nutrient-induced GLP-1, PYY and CCK release from isolated pig intestinal segments. Next, serotonin-induced GLP-1 release was studied in enteroendocrine STC-1 cells, where effects of serotonin receptor inhibition were studied using specific and non-specific antagonists. Casein (1% w/v), safflower oil (3.35% w/v), sucrose (50mM) and rebaudioside A (12.5mM) stimulated GLP-1 release from intestinal segments, whereas casein only stimulated PYY and CCK release. Combining nutrients with fluoxetine further increased nutrient-induced GLP-1, PYY and CCK release. Serotonin release from intestinal tissue segments was stimulated by casein and safflower oil while sucrose and rebaudioside A had no effect. The combination with fluoxetine (0.155µM) further enhanced casein and safflower oil induced-serotonin release. Exposure of ileal tissue segments to serotonin (30µM) stimulated GLP-1 release whereas it did not induce PYY and CCK release. Serotonin (30 and 100µM) also stimulated GLP-1 release from STC-1 cells, which was inhibited by the non-specific 5HT receptor antagonist asenapine (1 and 10µM). These data suggest that nutrient-induced GLP-1 release is modulated by serotonin through a receptor mediated process.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Edulcorantes / Aceite de Cártamo / Caseínas / Serotonina / Sacarosa en la Dieta / Células Enteroendocrinas / Péptido 1 Similar al Glucagón Límite: Animals Idioma: En Revista: J Nutr Biochem Asunto de la revista: BIOQUIMICA / CIENCIAS DA NUTRICAO Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Edulcorantes / Aceite de Cártamo / Caseínas / Serotonina / Sacarosa en la Dieta / Células Enteroendocrinas / Péptido 1 Similar al Glucagón Límite: Animals Idioma: En Revista: J Nutr Biochem Asunto de la revista: BIOQUIMICA / CIENCIAS DA NUTRICAO Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos