Transcriptional changes in steroidogenesis by perfluoroalkyl acids (PFOA and PFOS) regulate the synthesis of sex hormones in H295R cells.

Kang, Jae Soon; Choi, Jin-Soo; Park, June-Woo

Kang, Jae Soon; Choi, Jin-Soo; Park, June-Woo.

Afiliación

Kang JS; Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology, Jin-Ju, Gyeongnam, Republic of Korea.
Choi JS; Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology, Jin-Ju, Gyeongnam, Republic of Korea.
Park JW; Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology, Jin-Ju, Gyeongnam, Republic of Korea; Human and Environmental Toxicology Program, Korea University of Science and Technology (UST), Daejeon, Republic of Korea. Electronic address: jwpark@kitox.re.kr.

Chemosphere ; 155: 436-443, 2016 Jul.

Article en En | MEDLINE | ID: mdl-27139122

RESUMEN

Perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) are two of the most widely used perfluoroalkyl acids (PFAAs). Because of their strong persistence, they have become widely distributed throughout the environment and human bodies. PFOA and PFOS are suspected to disrupt the endocrine system based upon many in vivo studies, but the underlying mechanisms are currently unclear. In this study, we investigated the endocrine-related effects of PFOA and PFOS using in vitro estrogen receptor (ER) and androgen receptor (AR) transactivation assays and steroidogenesis assay. The results showed that PFOA and PFOS exhibited weak antagonistic ER transactivation but did not exhibit agonistic ER or AR transactivation. In the steroidogenesis assay, PFOA and PFOS induced 17ß-estradiol (E2) level and reduced testosterone level, which would be caused by the induction of aromatase activity. The qPCR analysis of genes involved in steroidogenesis indicates that PFOA and PFOS associate with sex hormone synthesis by the transcriptional induction of two genes, cyp19 and 3ß-hsd2. Moreover, the transcriptional induction of cyp11b2 by PFOS suggests that this chemical may underlie the disruption of several physiological functions related to aldosterone. The results of the current study suggest that PFOA and PFOS are potential endocrine disrupting chemicals (EDCs) and provide information for further studies on the molecular events that initiate the adverse endocrine effects.

Asunto(s)

Carcinoma Corticosuprarrenal/genética; Ácidos Alcanesulfónicos/farmacología; Caprilatos/farmacología; Disruptores Endocrinos/farmacología; Sistema Endocrino/efectos de los fármacos; Fluorocarburos/farmacología; Hormonas Esteroides Gonadales/metabolismo; Receptores Androgénicos/genética; Receptores de Estrógenos/genética; Carcinoma Corticosuprarrenal/metabolismo; Carcinoma Corticosuprarrenal/patología; Aromatasa/genética; Aromatasa/metabolismo; Bioensayo; Estrógenos/farmacología; Regulación Neoplásica de la Expresión Génica/efectos de los fármacos; Humanos; Reacción en Cadena en Tiempo Real de la Polimerasa; Receptores Androgénicos/metabolismo; Receptores de Estrógenos/metabolismo; Células Tumorales Cultivadas

Palabras clave

Aromatase; ER/AR transactivation; Endocrine disruptor chemical; Perfluorooctane sulfonate; Perfluorooctanoic acid; Steroidogenesis

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hormonas Esteroides Gonadales / Caprilatos / Receptores Androgénicos / Receptores de Estrógenos / Carcinoma Corticosuprarrenal / Ácidos Alcanesulfónicos / Sistema Endocrino / Disruptores Endocrinos / Fluorocarburos Límite: Humans Idioma: En Revista: Chemosphere Año: 2016 Tipo del documento: Article Pais de publicación: Reino Unido

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