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Active nano/microbilayer hemostatic agents for diabetic rat bleeding model.
Karahaliloglu, Zeynep; Demirbilek, Murat; Ulusoy, Ibrahim; Gümüskaya, Berrak; Denkbas, Emir Baki.
Afiliación
  • Karahaliloglu Z; Department of Biology, Faculty of Science and Arts, Aksaray University, Aksaray, Turkey.
  • Demirbilek M; Advanced Technologies Research and Application Center, Hacettepe University, Beytepe, Ankara, Turkey.
  • Ulusoy I; Adacell, Yildirim Beyazit Training and Research Hospital, Diskapi, Ankara, Turkey.
  • Gümüskaya B; Department of Pathology, Faculty of Medicine, Yildirim Beyazit University, Bilkent, Ankara, Turkey.
  • Denkbas EB; Department of Pathology, Atatürk Training and Research Hospital, Bilkent, Ankara, Turkey.
J Biomed Mater Res B Appl Biomater ; 105(6): 1573-1585, 2017 08.
Article en En | MEDLINE | ID: mdl-27126123
Patients with diabetes mellitus have an increased cardiovascular risk due to the abnormality of hemostatic system components. Therefore, hemostasis is an important concept when considering that diabetics are under risk due to potential bleeding complications during surgical operation. The aim of our study was to examine the efficiency of a fabricated nano/microbilayer hemostatic dressing for bleeding control in diabetic patients. For this purpose, we prepared a nano/microbilayer hemostatic dressing that has a porous sublayer, including chitosan (CTS), bacterial cellulose (BC) as basement and active agents in coagulation cascade, such as vitamin K (Vit K), protamine sulfate (PS), and kaolin (Kao) as a filler and an upper layer consisting of silk fibroin (SF) or SF/phosphatidylcholine (PC) blend to achieve complete hemostasis in diabetic rats. Coagulative performances of the prepared hemostatic dressings were examined by the determination of bleeding time, blood loss, and mortality rate through diabetic rat femoral artery injury model. The percent of diabetic rat blood absorption was found to be 247 ± 5% for gauze as opposed to 2214 ± 56% for SF-coated PS/BC/CTS. Vit K-reinforced within 138 s and SF-coated BC/CTS hemostatic dressings within 144 s showed a rapid coagulation time. In vivo coagulation studies demonstrated that hemostatic agent-reinforced BC/CTS hemostatic dressing, especially PS/BC/CTS showed a significant hemostatic plug formation. This study suggests that the high positive charge and porosity give to these hemostatic agents reinforced hemostatic dressings the ability to rapidly swell and to promote the accumulation of red blood cells and platelets through electrostatic interactions. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1573-1585, 2017.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vendajes / Hemostáticos / Arteria Femoral / Hemorragia Límite: Animals Idioma: En Revista: J Biomed Mater Res B Appl Biomater Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2017 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Vendajes / Hemostáticos / Arteria Femoral / Hemorragia Límite: Animals Idioma: En Revista: J Biomed Mater Res B Appl Biomater Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2017 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Estados Unidos