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Factors regulating bone remodeling processes in aseptic implant loosening.
Hartmann, Eliza S; Köhler, Miriam I; Huber, Felicitas; Redeker, Julia I; Schmitt, Baerbel; Schmitt-Sody, Marcus; Summer, Burkhard; Fottner, Andreas; Jansson, Volkmar; Mayer-Wagner, Susanne.
Afiliación
  • Hartmann ES; Department of Orthopaedic Surgery, Physical Medicine and Rehabilitation, Campus Großhadern, Ludwig-Maximilians-University, Marchioninistr 15, Munich 81377, Germany.
  • Köhler MI; Department of Orthopaedic Surgery, Physical Medicine and Rehabilitation, Campus Großhadern, Ludwig-Maximilians-University, Marchioninistr 15, Munich 81377, Germany.
  • Huber F; Department of Orthopaedic Surgery, Physical Medicine and Rehabilitation, Campus Großhadern, Ludwig-Maximilians-University, Marchioninistr 15, Munich 81377, Germany.
  • Redeker JI; Department of Orthopaedic Surgery, Physical Medicine and Rehabilitation, Campus Großhadern, Ludwig-Maximilians-University, Marchioninistr 15, Munich 81377, Germany.
  • Schmitt B; Department of Orthopaedic Surgery, Physical Medicine and Rehabilitation, Campus Großhadern, Ludwig-Maximilians-University, Marchioninistr 15, Munich 81377, Germany.
  • Schmitt-Sody M; Department of Orthopaedic Surgery, Physical Medicine and Rehabilitation, Campus Großhadern, Ludwig-Maximilians-University, Marchioninistr 15, Munich 81377, Germany.
  • Summer B; Department of Dermatology, Ludwig-Maximilians-University, Frauenlobstr 9-11, Munich 80337, Germany.
  • Fottner A; Department of Orthopaedic Surgery, Physical Medicine and Rehabilitation, Campus Großhadern, Ludwig-Maximilians-University, Marchioninistr 15, Munich 81377, Germany.
  • Jansson V; Department of Orthopaedic Surgery, Physical Medicine and Rehabilitation, Campus Großhadern, Ludwig-Maximilians-University, Marchioninistr 15, Munich 81377, Germany.
  • Mayer-Wagner S; Department of Orthopaedic Surgery, Physical Medicine and Rehabilitation, Campus Großhadern, Ludwig-Maximilians-University, Marchioninistr 15, Munich 81377, Germany.
J Orthop Res ; 35(2): 248-257, 2017 02.
Article en En | MEDLINE | ID: mdl-27116254
This study was undertaken to screen periprosthetic tissues (PPTs) under specified conditions for a series of molecular components and describe them in bone remodeling processes within aseptic loosening. PPT samples were obtained from patients undergoing revision surgery of endoprostheses (n = 24) and synovial tissues from patients with OA (control) (n = 18), patients with any form of inflammatory arthritides were excluded. Tissue samples were examined via microbiology, histology (H&E, TRAP), immunohistochemistry (CD68/anti-S100a4), quantitative real-time PCR (ALP, COL1A1, cathepsin K, M-CSF, MMP13, OPG, RANK, RANKL, TNF-α, and TRAP) and an endotoxin-assay. PPT samples contained a variety of cellular components and stained positive for TRAP (56%), CD68 (100%), and S100a4 (100%). Wear debris were found in cells staining positive for CD68 and S100a4. In PPTs significantly higher ALP, COL1A1, MMP-13, RANK, RANKL, and TRAP expression were found along with a significantly higher RANKL/OPG ratio and a significantly lower OPG expression. No significant difference was observed for M-CSF, TNF-α, cathepsin K, and endotoxin levels. In conclusion we found osteogenic proteins (ALP, COL1A1), a proteolytic enzyme (MMP-13), markers for osteoclast differentiation (RANK, RANKL), and osteoclast activity (TRAP) to be increased in PPT, whereas OPG expression decreased significantly in comparison to control. We present data about a large series of molecular components in PPT and describe novel and key findings about their expression levels in regards to aseptic implant loosening. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:248-257, 2017.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Huesos / Falla de Prótesis / Remodelación Ósea Tipo de estudio: Observational_studies Límite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: J Orthop Res Año: 2017 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Huesos / Falla de Prótesis / Remodelación Ósea Tipo de estudio: Observational_studies Límite: Aged / Aged80 / Female / Humans / Male / Middle aged Idioma: En Revista: J Orthop Res Año: 2017 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos