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Induction of expression and phosphorylation of heat shock protein B5 (CRYAB) in rat myometrium during pregnancy and labour.
Nicoletti, J G; White, B G; Miskiewicz, E I; MacPhee, D J.
Afiliación
  • Nicoletti JG; Department of Veterinary Biomedical SciencesWestern College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada One Reproductive Health Research GroupUniversity of Saskatchewan, Saskatoon, Saskatchewan, Canada.
  • White BG; Okanagan CollegeSalmon Arm Campus, Salmon Arm, British Columbia, Canada.
  • Miskiewicz EI; Department of Veterinary Biomedical SciencesWestern College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada One Reproductive Health Research GroupUniversity of Saskatchewan, Saskatoon, Saskatchewan, Canada.
  • MacPhee DJ; Department of Veterinary Biomedical SciencesWestern College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada One Reproductive Health Research GroupUniversity of Saskatchewan, Saskatoon, Saskatchewan, Canada Division of Biomedical SciencesFaculty of Medicine, Memori
Reproduction ; 152(1): 69-79, 2016 07.
Article en En | MEDLINE | ID: mdl-27107034
During pregnancy the myometrium undergoes a programme of differentiation induced by endocrine, cellular, and biophysical inputs. Small heat shock proteins (HSPs) are a family of ten (B1-B10) small-molecular-weight proteins that not only act as chaperones, but also assist in processes such as cytoskeleton rearrangements and immune system activation. Thus, it was hypothesized that HSPB5 (CRYAB) would be highly expressed in the rat myometrium during the contractile and labour phases of myometrial differentiation when such processes are prominent. Immunoblot analysis revealed that myometrial CRYAB protein expression significantly increased from day (D) 15 to D23 (labour; P<0.05). In correlation with these findings, serine 59-phosphorylated (pSer59) CRYAB protein expression significantly increased from D15 to D23, and was also elevated 1-day post-partum (P<0.05). pSer59-CRYAB was detected in the cytoplasm of myocytes within both uterine muscle layers mid- to late-pregnancy. In unilaterally pregnant rats, pSer59-CRYAB protein expression was significantly elevated in the gravid uterine horns at both D19 and D23 of gestation compared with non-gravid horns. Co-immunolocalization experiments using the hTERT-human myometrial cell line and confocal microscopy demonstrated that pSer59-CRYAB co-localized with the focal adhesion protein FERMT2 at the ends of actin filaments as well as with the exosomal marker CD63. Overall, pSer59-CRYAB is highly expressed in myometrium during late pregnancy and labour and its expression appears to be regulated by uterine distension. CRYAB may be involved in the regulation of actin filament dynamics at focal adhesions and could be secreted by exosomes as a prelude to involvement in immune activation in the myometrium.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Útero / Trabajo de Parto / Preñez / Cristalinas / Proteínas Asociadas a Microtúbulos / Músculo Liso / Miometrio Límite: Animals / Female / Humans / Pregnancy Idioma: En Revista: Reproduction Asunto de la revista: MEDICINA REPRODUTIVA Año: 2016 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Útero / Trabajo de Parto / Preñez / Cristalinas / Proteínas Asociadas a Microtúbulos / Músculo Liso / Miometrio Límite: Animals / Female / Humans / Pregnancy Idioma: En Revista: Reproduction Asunto de la revista: MEDICINA REPRODUTIVA Año: 2016 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Reino Unido