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Segregation of nod-containing and nod-deficient bradyrhizobia as endosymbionts of Arachis hypogaea and as endophytes of Oryza sativa in intercropped fields of Bengal Basin, India.
Guha, Sohini; Sarkar, Monolina; Ganguly, Pritha; Uddin, Md Raihan; Mandal, Sukhendu; DasGupta, Maitrayee.
Afiliación
  • Guha S; Department of Biochemistry, University of Calcutta, Kolkata, 700019, India.
  • Sarkar M; Department of Biochemistry, University of Calcutta, Kolkata, 700019, India.
  • Ganguly P; Department of Biochemistry, University of Calcutta, Kolkata, 700019, India.
  • Uddin MR; Department of Microbiology, University of Calcutta, Kolkata, 700019, India.
  • Mandal S; Department of Microbiology, University of Calcutta, Kolkata, 700019, India.
  • DasGupta M; Department of Biochemistry, University of Calcutta, Kolkata, 700019, India.
Environ Microbiol ; 18(8): 2575-90, 2016 09.
Article en En | MEDLINE | ID: mdl-27102878
Bradyrhizobial invasion in dalbergoid legumes like Arachis hypogaea and endophytic bacterial invasions in non-legumes like Oryza sativa occur through epidermal cracks. Here, we show that there is no overlap between the bradyrhizobial consortia that endosymbiotically and endophytically colonise these plants. To minimise contrast due to phylogeographic isolation, strains were collected from Arachis/Oryza intercropped fields and a total of 17 bradyrhizobia from Arachis (WBAH) and 13 from Oryza (WBOS) were investigated. 16SrRNA and concatenated dnaK-glnII-recA phylogeny clustered the nodABC-positive WBAH and nodABC-deficient WBOS strains in two distinct clades. The in-field segregation is reproducible under controlled conditions which limits the factors that influence their competitive exclusion. While WBAH renodulated Arachis successfully, WBOS nodulated in an inefficient manner. Thus, Arachis, like other Aeschynomene legumes support nod-independent symbiosis that was ineffectual in natural fields. In Oryza, WBOS recolonised endophytically and promoted its growth. WBAH however caused severe chlorosis that was completely overcome when coinfected with WBOS. This explains the exclusive recovery of WBOS in Oryza in natural fields and suggests Nod-factors to have a role in counterselection of WBAH. Finally, canonical soxY1 and thiosulphate oxidation could only be detected in WBOS indicating loss of metabolic traits in WBAH with adaptation of symbiotic lifestyle.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arachis / Oryza / Simbiosis / Bradyrhizobium / Endófitos País/Región como asunto: Asia Idioma: En Revista: Environ Microbiol Asunto de la revista: MICROBIOLOGIA / SAUDE AMBIENTAL Año: 2016 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arachis / Oryza / Simbiosis / Bradyrhizobium / Endófitos País/Región como asunto: Asia Idioma: En Revista: Environ Microbiol Asunto de la revista: MICROBIOLOGIA / SAUDE AMBIENTAL Año: 2016 Tipo del documento: Article País de afiliación: India Pais de publicación: Reino Unido