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A New Carbon Nanotube-Based Breast Cancer Drug Delivery System: Preparation and In Vitro Analysis Using Paclitaxel.
Shao, Wei; Paul, Arghya; Rodes, Laetitia; Prakash, Satya.
Afiliación
  • Shao W; Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, 3775 University Street, Montreal, QC, H3A 2B4, Canada.
  • Paul A; Department of Chemical and Petroleum Engineering, University of Kansas, Learned Hall 1530 W 15th St, Lawrence, KS, 66045, USA.
  • Rodes L; Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, 3775 University Street, Montreal, QC, H3A 2B4, Canada.
  • Prakash S; Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, 3775 University Street, Montreal, QC, H3A 2B4, Canada. satya.prakash@mcgill.ca.
Cell Biochem Biophys ; 71(3): 1405-14, 2015 Apr.
Article en En | MEDLINE | ID: mdl-27101155
Paclitaxel (PTX) is one of the most important drugs for breast cancer; however, the drug effects are limited by its systematic toxicity and poor water solubility. Nanoparticles have been applied for delivery of cancer drugs to overcome their limitations. Toward this goal, a novel single-walled carbon nanotube (SWNT)-based drug delivery system was developed by conjugation of human serum albumin (HSA) nanoparticles for loading of antitumor agent PTX. The nanosized macromolecular SWNT-drug carrier (SWNT-HSA) was characterized by TEM, UV-Vis-NIR spectrometry, and TGA. The SWNT-based drug carrier displayed high intracellular delivery efficiency (cell uptake rate of 80%) in breast cancer MCF-7 cells, as examined by fluorescence-labeled drug carriers, suggesting the needle-shaped SWNT-HSA drug carrier was able to transport drugs across cell membrane despite its macromolecular structure. The drug loading on SWNT-based drug carrier was through high binding affinity of PTX to HSA proteins. The PTX formulated with SWNT-HSA showed greater growth inhibition activity in MCF-7 breast cancer cells than PTX formulated with HSA nanoparticle only (cell viability of 63 vs 70% in 48 h and 53 vs 62% in 72 h). The increased drug efficacy could be driven by SWNT-mediated cell internalization. These data suggest that the developed SWNT-based antitumor agent is functional and effective. However, more studies for in vivo drug delivery efficacy and other properties are needed before this delivery system can be fully realized.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Portadores de Fármacos / Paclitaxel / Nanotubos de Carbono / Antineoplásicos Límite: Humans Idioma: En Revista: Cell Biochem Biophys Asunto de la revista: BIOFISICA / BIOQUIMICA Año: 2015 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Mama / Portadores de Fármacos / Paclitaxel / Nanotubos de Carbono / Antineoplásicos Límite: Humans Idioma: En Revista: Cell Biochem Biophys Asunto de la revista: BIOFISICA / BIOQUIMICA Año: 2015 Tipo del documento: Article País de afiliación: Canadá Pais de publicación: Estados Unidos