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The Role of miR-34a in Tritiated Water Toxicity in Human Umbilical Vein Endothelial Cells.
Cui, Feng Mei; Liu, Liang; Zheng, Lu Lin; Bao, Guang Liang; Tu, Yu; Sun, Liang; Zhu, Wei; Cao, Jian Ping; Zhou, Ping Kun; Chen, Qiu; He, Yong Ming.
Afiliación
  • Cui FM; Department of Radiation Medicine, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China; Collaborative Innovation Center of Radiation Medicine, Jiangsu Higher Education Institutions, Suzhou, China.
  • Liu L; Department of Cardiology, the First Affiliated Hospital of Soochow University, Suzhou, China.
  • Zheng LL; Department of Radiation Medicine, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China.
  • Bao GL; Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.
  • Tu Y; Department of Radiation Medicine, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China; Collaborative Innovation Center of Radiation Medicine, Jiangsu Higher Education Institutions, Suzhou, China.
  • Sun L; Department of Radiation Medicine, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China; Collaborative Innovation Center of Radiation Medicine, Jiangsu Higher Education Institutions, Suzhou, China.
  • Zhu W; Department of Radiation Medicine, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China; Collaborative Innovation Center of Radiation Medicine, Jiangsu Higher Education Institutions, Suzhou, China.
  • Cao JP; Department of Radiation Medicine, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China; Collaborative Innovation Center of Radiation Medicine, Jiangsu Higher Education Institutions, Suzhou, China.
  • Zhou PK; Department of Radiation Medicine, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China; Collaborative Innovation Center of Radiation Medicine, Jiangsu Higher Education Institutions, Suzhou, China.
  • Chen Q; Department of Radiation Medicine, School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China; Collaborative Innovation Center of Radiation Medicine, Jiangsu Higher Education Institutions, Suzhou, China.
  • He YM; Department of Cardiology, the First Affiliated Hospital of Soochow University, Suzhou, China.
Dose Response ; 14(2): 1559325816638585, 2016.
Article en En | MEDLINE | ID: mdl-27099602
In this work, we investigated the toxic effects of tritiated water (HTO) on the cardiovascular system. We examined the role of microRNA-34a (miR-34a) in DNA damage and repair in human umbilical vein endothelial cells (HUVECs) exposed to HTO. Cell proliferation capacity was evaluated by cell counting, and miR-34a expression was detected using quantitative PCR (QT-PCR). The Comet assay and γ-H2AX immunostaining were used to measure DNA double-strand breaks (DSBs). Reverse transcription polymerase chain reaction was used to measure the expression level of c-myc messenger RNA (mRNA). The cells exposed to HTO showed significantly lower proliferation than the control cells over 3 days. The DNA damage in the HTO group was more severe than that in the control group, at each time point examined. The expression of miR-34a mimics caused increased DNA DSBs whereas that of the miR-34a inhibitor caused decreased DNA DSBs. The proliferation viability was the opposite for the miR-34a mimics and inhibitor groups. The expression levels of c-myc mRNA in cells transfected with miR-34a mimics were lower than that in cells transfected with the miR-34a-5p inhibitor, at 0.5 hours and 2 hours after transfection. In summary, miR-34a mediates HTO toxicity in HUVECs by downregulating the expression of c-myc.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Dose Response Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Dose Response Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos