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Endometrial transcriptome analysis indicates superiority of natural over artificial cycles in recurrent implantation failure patients undergoing frozen embryo transfer.
Altmäe, Signe; Tamm-Rosenstein, Karin; Esteban, Francisco J; Simm, Jaak; Kolberg, Liis; Peterson, Hedi; Metsis, Madis; Haldre, Kai; Horcajadas, José A; Salumets, Andres; Stavreus-Evers, Anneli.
Afiliación
  • Altmäe S; Competence Centre on Health Technologies, Tartu, Estonia; Department of Paediatrics, School of Medicine, University of Granada, Granada, Spain. Electronic address: signealtmae@ugr.es.
  • Tamm-Rosenstein K; Department of Gene Technology, Tallinn University of Technology, Tallinn, Estonia.
  • Esteban FJ; Department of Experimental Biology, University of Jaén, Jaén, Spain.
  • Simm J; Department of Gene Technology, Tallinn University of Technology, Tallinn, Estonia.
  • Kolberg L; Institute of Computer Science, University of Tartu, Estonia.
  • Peterson H; Institute of Computer Science, University of Tartu, Estonia; Quretec Ltd., Tartu, Estonia.
  • Metsis M; Competence Centre on Health Technologies, Tartu, Estonia; School of Natural Sciences and Health, Tallinn University, Tallinn, Estonia.
  • Haldre K; Centre for Reproductive Medicine, West Tallinn Central Hospital Women's Clinic, Tallinn, Estonia.
  • Horcajadas JA; Araid at I + CS, Hospital Miguel Servet, Zaragoza, Spain.
  • Salumets A; Competence Centre on Health Technologies, Tartu, Estonia; Department of Obstetrics and Gynaecology, University of Tartu, Tartu, Estonia.
  • Stavreus-Evers A; Department of Women's and Children's Health, Uppsala University, Uppsala, Sweden.
Reprod Biomed Online ; 32(6): 597-613, 2016 Jun.
Article en En | MEDLINE | ID: mdl-27090967
Little consensus has been reached on the best protocol for endometrial preparation for frozen embryo transfer (FET). It is not known how, and to what extent, hormone supplementation in artificial cycles influences endometrial preparation for embryo implantation at a molecular level, especially in patients who have experienced recurrent implantation failure. Transcriptome analysis of 15 endometrial biopsy samples at the time of embryo implantation was used to compare two different endometrial preparation protocols, natural versus artificial cycles, for FET in women who have experienced recurrent implantation failure compared with fertile women. IPA and DAVID were used for functional analyses of differentially expressed genes. The TRANSFAC database was used to identify oestrogen and progesterone response elements upstream of differentially expressed genes. Cluster analysis demonstrated that natural cycles are associated with a better endometrial receptivity transcriptome than artificial cycles. Artificial cycles seemed to have a stronger negative effect on expression of genes and pathways crucial for endometrial receptivity, including ESR2, FSHR, LEP, and several interleukins and matrix metalloproteinases. Significant overrepresentation of oestrogen response elements among the genes with deteriorated expression in artificial cycles (P < 0.001) was found; progesterone response elements predominated in genes with amended expression with artificial cycles (P = 0.0052).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Implantación del Embrión / Transferencia de Embrión / Endometrio Límite: Adult / Female / Humans / Pregnancy Idioma: En Revista: Reprod Biomed Online Asunto de la revista: MEDICINA REPRODUTIVA Año: 2016 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Implantación del Embrión / Transferencia de Embrión / Endometrio Límite: Adult / Female / Humans / Pregnancy Idioma: En Revista: Reprod Biomed Online Asunto de la revista: MEDICINA REPRODUTIVA Año: 2016 Tipo del documento: Article Pais de publicación: Países Bajos