Your browser doesn't support javascript.
loading
A lectin-based cell microarray approach to analyze the mammalian granulosa cell surface glycosylation profile.
Accogli, Gianluca; Desantis, Salvatore; Martino, Nicola Antonio; Dell'Aquila, Maria Elena; Gemeiner, Peter; Katrlík, Jaroslav.
Afiliación
  • Accogli G; Department of Emergency and Organ Transplantation (DETO), Veterinary Clinics and Animal Productions Unit, University of Bari Aldo Moro, SP Casamassima, km 3, 70010, Valenzano, Bari, Italy.
  • Desantis S; Department of Emergency and Organ Transplantation (DETO), Veterinary Clinics and Animal Productions Unit, University of Bari Aldo Moro, SP Casamassima, km 3, 70010, Valenzano, Bari, Italy.
  • Martino NA; Department of Biosciences, Biotechnologies and Biopharmaceutics (DBBB), University of Bari Aldo Moro, SP Casamassima, km 3, 70010, Valenzano, Bari, Italy.
  • Dell'Aquila ME; Experimental Zooprophylactic Institute of Puglia and Basilicata, Via Manfredonia 20, 71121, Foggia, Italy.
  • Gemeiner P; Department of Biosciences, Biotechnologies and Biopharmaceutics (DBBB), University of Bari Aldo Moro, SP Casamassima, km 3, 70010, Valenzano, Bari, Italy.
  • Katrlík J; Department of Glycobiotechnology, Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84538, Bratislava, Slovakia.
Glycoconj J ; 33(5): 717-24, 2016 10.
Article en En | MEDLINE | ID: mdl-27085877
The high complexity of glycome, the repertoire of glycans expressed in a cell or in an organism, is difficult to analyze and the use of new technologies has accelerated the progress of glycomics analysis. In the last decade, the microarray approaches, and in particular glycan and lectin microarrays, have provided new insights into evaluation of cell glycosylation status. Here we present a cell microarray method based on cell printing on microarray slides for the analysis of the glycosylation pattern of the cell glycocalyx. In order to demonstrate the reliability of the developed method, the glycome profiles of equine native uncultured mural granulosa cells (uGCs) and in vitro cultured mural granulosa cells (cGCs) were determined and compared. The method consists in the isolation of GCs, cell printing into arrays on microarray slide, incubation with a panel of biotinylated lectins, reaction with fluorescent streptavidin and signal intensity detection by a microarray scanner. Cell microarray technology revealed that glycocalyx of both uGCs and cGCs contains N-glycans, sialic acid terminating glycans, N-acetylglucosamine and O-glycans. The comparison of uGCs and cGCs glycan signals indicated an increase in the expression of sialic acids, N-acetylglucosamine, and N-glycans in cGCs. Glycan profiles determined by cell microarray agreed with those revealed by lectin histochemistry. The described cell microarray method represents a simple and sensitive procedure to analyze cell surface glycome in mammalian cells.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicocálix / Análisis de Matrices Tisulares / Células de la Granulosa / Lectinas Límite: Animals Idioma: En Revista: Glycoconj J Asunto de la revista: BIOQUIMICA / METABOLISMO Año: 2016 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicocálix / Análisis de Matrices Tisulares / Células de la Granulosa / Lectinas Límite: Animals Idioma: En Revista: Glycoconj J Asunto de la revista: BIOQUIMICA / METABOLISMO Año: 2016 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos