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Excretory/secretory products of adult Haemonchus contortus and Teladorsagia circumcincta which increase the permeability of Caco-2 cell monolayers are neutralised by antibodies from immune hosts.
Rehman, Z U; Deng, Q; Umair, S; Savoian, M S; Knight, J S; Pernthaner, A; Simpson, H V.
Afiliación
  • Rehman ZU; Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Private Bag 11-222, Palmerston North, New Zealand.
  • Deng Q; AgResearch Ltd., Private Bag 11-008, Palmerston North, New Zealand.
  • Umair S; AgResearch Ltd., Private Bag 11-008, Palmerston North, New Zealand.
  • Savoian MS; Institute of Fundamental Sciences, Massey University, Private Bag 11-222, Palmerston North, New Zealand.
  • Knight JS; AgResearch Ltd., Private Bag 11-008, Palmerston North, New Zealand.
  • Pernthaner A; AgResearch Ltd., Private Bag 11-008, Palmerston North, New Zealand.
  • Simpson HV; Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Private Bag 11-222, Palmerston North, New Zealand. Electronic address: H.V.Simpson@massey.ac.nz.
Vet Parasitol ; 221: 104-10, 2016 May 15.
Article en En | MEDLINE | ID: mdl-27084480
The onset of abomasal pathophysiology due to parasitism coincides with the presence of adult worms in the lumen, implicating worm excretory/secretory (ES) products acting on the surface mucosa. Caco-2 cell monolayers were grown to confluence on Transwell plates and exposed on the apical side to ES products of adult Haemonchus contortus and Teladorsagia circumcincta. ES products of both species significantly (p<0.001) reduced transepithelial electrical resistance after 2h to 81.1±1.0% and 82.9±1.1% respectively. Immunocytochemical staining of the Caco-2 monolayers for zona occludens-1 and occludin confirmed that the tight junctions remained intact in control medium, but these proteins were internalised from disrupted junctions after exposure to ES products. The components of H. contortus ES products responsible for increased epithelial permeability were partially blocked by phage displaying single chain antibodies derived from sheep immune to field infection and enriched by panning with H. contortus ES products. Immune hosts may therefore be able to reduce the effects of worm chemicals on the gastric epithelium. Permeabilisation of the abomasal surface mucosa by worm chemicals would also explain how cells deep in the gastric glands could rapidly be affected by parasites emerging from the glands or within a day of transplantation of adult worms into naïve hosts, resulting in the pathophysiology typically caused by abomasal nematode parasitism.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anticuerpos Antihelmínticos / Proteínas del Helminto / Anticuerpos Neutralizantes / Haemonchus / Interacciones Huésped-Parásitos Límite: Animals / Humans Idioma: En Revista: Vet Parasitol Año: 2016 Tipo del documento: Article País de afiliación: Nueva Zelanda Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Anticuerpos Antihelmínticos / Proteínas del Helminto / Anticuerpos Neutralizantes / Haemonchus / Interacciones Huésped-Parásitos Límite: Animals / Humans Idioma: En Revista: Vet Parasitol Año: 2016 Tipo del documento: Article País de afiliación: Nueva Zelanda Pais de publicación: Países Bajos