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Single cell analysis in native tissue: Quantification of the retinoid content of hepatic stellate cells.
Galler, Kerstin; Requardt, Robert Pascal; Glaser, Uwe; Markwart, Robby; Bocklitz, Thomas; Bauer, Michael; Popp, Jürgen; Neugebauer, Ute.
Afiliación
  • Galler K; Leibniz Institute of Photonic Technology, Jena, Germany.
  • Requardt RP; Center for Sepsis Control and Care, Jena University Hospital, Germany.
  • Glaser U; Center for Sepsis Control and Care, Jena University Hospital, Germany.
  • Markwart R; Leibniz Institute of Photonic Technology, Jena, Germany.
  • Bocklitz T; Center for Sepsis Control and Care, Jena University Hospital, Germany.
  • Bauer M; Center for Sepsis Control and Care, Jena University Hospital, Germany.
  • Popp J; Leibniz Institute of Photonic Technology, Jena, Germany.
  • Neugebauer U; Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, Germany.
Sci Rep ; 6: 24155, 2016 Apr 11.
Article en En | MEDLINE | ID: mdl-27063397
Hepatic stellate cells (HSCs) are retinoid storing cells in the liver: The retinoid content of those cells changes depending on nutrition and stress level. There are also differences with regard to a HSC's anatomical position in the liver. Up to now, retinoid levels were only accessible from bulk measurements of tissue homogenates or cell extracts. Unfortunately, they do not account for the intercellular variability. Herein, Raman spectroscopy relying on excitation by the minimally destructive wavelength 785 nm is introduced for the assessment of the retinoid state of single HSCs in freshly isolated, unprocessed murine liver lobes. A quantitative estimation of the cellular retinoid content is derived. Implications of the retinoid content on hepatic health state are reported. The Raman-based results are integrated with histological assessments of the tissue samples. This spectroscopic approach enables single cell analysis regarding an important cellular feature in unharmed tissue.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Retinoides / Espectrometría Raman / Células Estrelladas Hepáticas Límite: Animals Idioma: En Revista: Sci Rep Año: 2016 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Retinoides / Espectrometría Raman / Células Estrelladas Hepáticas Límite: Animals Idioma: En Revista: Sci Rep Año: 2016 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Reino Unido