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Influence of different buffers (HEPES/MOPS) on keratinocyte cell viability and microbial growth.
Dias, Kássia de Carvalho; Barbugli, Paula Aboud; Vergani, Carlos Eduardo.
Afiliación
  • Dias Kde C; Department of Dental Materials and Prosthodontics, Oral Rehabilitation Program-Araraquara School of Dentistry, UNESP-Univ. Estadual Paulista, Centro, 14801903 Araraquara, SP, Brazil. Electronic address: kassiaodonto@hotmail.com.
  • Barbugli PA; Department of Dental Materials and Prosthodontics, Oral Rehabilitation Program-Araraquara School of Dentistry, UNESP-Univ. Estadual Paulista, Centro, 14801903 Araraquara, SP, Brazil. Electronic address: pabfarma@yahoo.com.br.
  • Vergani CE; Department of Dental Materials and Prosthodontics, Oral Rehabilitation Program-Araraquara School of Dentistry, UNESP-Univ. Estadual Paulista, Centro, 14801903 Araraquara, SP, Brazil. Electronic address: vergani@foar.unesp.br.
J Microbiol Methods ; 125: 40-2, 2016 06.
Article en En | MEDLINE | ID: mdl-27060444
This study assessed the effect of the buffers 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and 3-(N-morpholino) propanesulfonic acid (MOPS) on keratinocyte cell viability and microbial growth. It was observed that RPMI buffered with HEPES, supplemented with l-glutamine and sodium bicarbonate, can be used as a more suitable medium to promote co-culture.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Staphylococcus aureus / Queratinocitos / Supervivencia Celular / Morfolinas / Medios de Cultivo / HEPES Límite: Animals / Humans Idioma: En Revista: J Microbiol Methods Año: 2016 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
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Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Staphylococcus aureus / Queratinocitos / Supervivencia Celular / Morfolinas / Medios de Cultivo / HEPES Límite: Animals / Humans Idioma: En Revista: J Microbiol Methods Año: 2016 Tipo del documento: Article Pais de publicación: Países Bajos