Your browser doesn't support javascript.
loading
Nascent DNA Proteomics Reveals a Chromatin Remodeler Required for Topoisomerase I Loading at Replication Forks.
Ribeyre, Cyril; Zellweger, Ralph; Chauvin, Maeva; Bec, Nicole; Larroque, Christian; Lopes, Massimo; Constantinou, Angelos.
Afiliación
  • Ribeyre C; Institute of Human Genetics, Centre National de la Recherche Scientifique (CNRS) UPR 1142, University of Montpellier, 34396 Montpellier, France. Electronic address: cyril.ribeyre@igh.cnrs.fr.
  • Zellweger R; Institute of Molecular Cancer Research, University of Zurich, 8057 Zurich, Switzerland.
  • Chauvin M; Institute of Human Genetics, Centre National de la Recherche Scientifique (CNRS) UPR 1142, University of Montpellier, 34396 Montpellier, France.
  • Bec N; Institut de Recherche en Cancérologie de Montpellier, INSERM, U1194, University of Montpellier, 34298 Montpellier, France.
  • Larroque C; Institut de Recherche en Cancérologie de Montpellier, INSERM, U1194, University of Montpellier, 34298 Montpellier, France.
  • Lopes M; Institute of Molecular Cancer Research, University of Zurich, 8057 Zurich, Switzerland.
  • Constantinou A; Institute of Human Genetics, Centre National de la Recherche Scientifique (CNRS) UPR 1142, University of Montpellier, 34396 Montpellier, France. Electronic address: angelos.constantinou@igh.cnrs.fr.
Cell Rep ; 15(2): 300-9, 2016 Apr 12.
Article en En | MEDLINE | ID: mdl-27050524
During transcription and DNA replication, the DNA template is overwound ahead of RNA and DNA polymerases and relaxed by DNA topoisomerases. Inhibitors of topoisomerases are potent anti-cancer agents. Camptothecin traps topoisomerase I on DNA and exerts preferential cytotoxicity toward cancer cells by way of its interference with the progression of replication forks. Starting with an unbiased proteomic analysis, we find that the chromatin remodeling complex BAZ1B-SMARCA5 accumulates near replication forks in camptothecin-exposed cells. We report that BAZ1B associates with topoisomerase I and facilitates its access to replication forks. Single-molecule analyses of replication structures show that BAZ1B contributes to replication interference by camptothecin. A lack of BAZ1B confers increased cellular tolerance of camptothecin. These findings reveal BAZ1B as a key facilitator of topoisomerase I function during DNA replication that affects the response of cancer cells to topoisomerase I inhibitors.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / ADN-Topoisomerasas de Tipo I / Proteómica / Ensamble y Desensamble de Cromatina / Replicación del ADN Tipo de estudio: Prognostic_studies Límite: Humans / Male Idioma: En Revista: Cell Rep Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / ADN-Topoisomerasas de Tipo I / Proteómica / Ensamble y Desensamble de Cromatina / Replicación del ADN Tipo de estudio: Prognostic_studies Límite: Humans / Male Idioma: En Revista: Cell Rep Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos