Your browser doesn't support javascript.
loading
A fully validated LC-MS/MS method for simultaneous determination of nicotine and its metabolite cotinine in human serum and its application to a pharmacokinetic study after using nicotine transdermal delivery systems with standard heat application in adult smokers.
Abdallah, Inas A; Hammell, Dana C; Stinchcomb, Audra L; Hassan, Hazem E.
Afiliación
  • Abdallah IA; Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, MD, United States; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Misr International University, Cairo, Egypt.
  • Hammell DC; Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, MD, United States.
  • Stinchcomb AL; Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, MD, United States. Electronic address: astinchc@rx.umaryland.edu.
  • Hassan HE; Department of Pharmaceutical Sciences, University of Maryland School of Pharmacy, Baltimore, MD, United States; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Helwan University, Cairo, Egypt. Electronic address: hhassan@rx.umaryland.edu.
J Chromatogr B Analyt Technol Biomed Life Sci ; 1020: 67-77, 2016 May 01.
Article en En | MEDLINE | ID: mdl-27023159
A sensitive and simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for simultaneous determination of nicotine and its main metabolite cotinine in human serum samples. Liquid-liquid extraction using ethyl acetate was employed for serum sample extractions. Chromatographic separation was achieved on Phenomenex Luna(®) HILIC column (150 mm x 3.0mm, 5 µm). Isocratic elution was performed using acetonitrile:100mM ammonium formate buffer (pH=3.2) (90:10, v/v) as the mobile phase, at a flow rate of 0.4 mL/min. Tandem mass spectrometric detection was employed at positive electrospray ionization in MRM mode for the determination of both nicotine and cotinine and their stable isotope labeled internal standards. Analysis was carried out in 8 min over a concentration range of 0.26-52.5 ng/mL and 7.0-1500 ng/mL for nicotine and cotinine, respectively. The assay was validated according to FDA guidelines for bioanalytical method validation and satisfactory results were obtained; the accuracy ranged between 93.39% and 105.73% for nicotine and between 93.04% and 107.26% for cotinine. No significant matrix effect was observed. Stability assays indicated both nicotine and cotinine were stable during sample storage, preparation and analytical procedures. The method was successfully applied to biological samples obtained from a pharmacokinetic study conducted in adult smokers to investigate heat effect on nicotine and cotinine serum levels after nicotine transdermal delivery system (TDS) application.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromatografía Liquida / Cotinina / Espectrometría de Masas en Tándem / Dispositivos para Dejar de Fumar Tabaco / Hipertermia Inducida / Nicotina Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2016 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromatografía Liquida / Cotinina / Espectrometría de Masas en Tándem / Dispositivos para Dejar de Fumar Tabaco / Hipertermia Inducida / Nicotina Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2016 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Países Bajos