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A Positive Feed-forward Loop Associating EGR1 and PDGFA Promotes Proliferation and Self-renewal in Glioblastoma Stem Cells.
Sakakini, Nathalie; Turchi, Laurent; Bergon, Aurélie; Holota, Hélène; Rekima, Samah; Lopez, Fabrice; Paquis, Philipe; Almairac, Fabien; Fontaine, Denys; Baeza-Kallee, Nathalie; Van Obberghen-Schilling, Ellen; Junier, Marie-Pierre; Chneiweiss, Hervé; Figarella-Branger, Dominique; Burel-Vandenbos, Fanny; Imbert, Jean; Virolle, Thierry.
Afiliación
  • Sakakini N; From the Université Nice Sophia Antipolis, CNRS, INSERM, iBV, 06108 Nice, France, INSERM, U1090, Transcriptomic and Genomic Marseille-Luminy/Technical Advances for Genomics and Clinics (TGML/TAGC), Marseille F-13009, France, UMR_S 1090, TGML/TAGC, Aix-Marseille Université, Marseille F-13009, France.
  • Turchi L; From the Université Nice Sophia Antipolis, CNRS, INSERM, iBV, 06108 Nice, France.
  • Bergon A; INSERM, U1090, Transcriptomic and Genomic Marseille-Luminy/Technical Advances for Genomics and Clinics (TGML/TAGC), Marseille F-13009, France, UMR_S 1090, TGML/TAGC, Aix-Marseille Université, Marseille F-13009, France.
  • Holota H; INSERM, U1090, Transcriptomic and Genomic Marseille-Luminy/Technical Advances for Genomics and Clinics (TGML/TAGC), Marseille F-13009, France, UMR_S 1090, TGML/TAGC, Aix-Marseille Université, Marseille F-13009, France.
  • Rekima S; From the Université Nice Sophia Antipolis, CNRS, INSERM, iBV, 06108 Nice, France.
  • Lopez F; INSERM, U1090, Transcriptomic and Genomic Marseille-Luminy/Technical Advances for Genomics and Clinics (TGML/TAGC), Marseille F-13009, France, UMR_S 1090, TGML/TAGC, Aix-Marseille Université, Marseille F-13009, France.
  • Paquis P; From the Université Nice Sophia Antipolis, CNRS, INSERM, iBV, 06108 Nice, France, the Service de Neurchirurgie, Hôpital Pasteur, CHU de Nice, Nice 06107, France.
  • Almairac F; From the Université Nice Sophia Antipolis, CNRS, INSERM, iBV, 06108 Nice, France, the Service de Neurchirurgie, Hôpital Pasteur, CHU de Nice, Nice 06107, France.
  • Fontaine D; the Service de Neurchirurgie, Hôpital Pasteur, CHU de Nice, Nice 06107, France.
  • Baeza-Kallee N; Aix Marseille Université, Faculté de Médecine de la Timone, 13284 Marseille, France, CRO2, INSERM UMR 911, 13284 Marseille Cedex, France.
  • Van Obberghen-Schilling E; From the Université Nice Sophia Antipolis, CNRS, INSERM, iBV, 06108 Nice, France.
  • Junier MP; CNRS UMR8246 Neuroscience Paris Seine-IBPS, Team Glial Plasticity, 7 Quai Saint-Bernard, Paris 75005, France, INSERM U1130, Neuroscience Paris Seine-IBPS, Team Glial Plasticity, 7 Quai Saint-Bernard, Paris 75005, France, and University Pierre and Marie Curie UMCR18, Neuroscience Paris Seine-IBPS, Te
  • Chneiweiss H; CNRS UMR8246 Neuroscience Paris Seine-IBPS, Team Glial Plasticity, 7 Quai Saint-Bernard, Paris 75005, France, INSERM U1130, Neuroscience Paris Seine-IBPS, Team Glial Plasticity, 7 Quai Saint-Bernard, Paris 75005, France, and University Pierre and Marie Curie UMCR18, Neuroscience Paris Seine-IBPS, Te
  • Figarella-Branger D; Aix Marseille Université, Faculté de Médecine de la Timone, 13284 Marseille, France, CRO2, INSERM UMR 911, 13284 Marseille Cedex, France, the Departement de Pathology, CHU de la Timone, 13385 Marseille Cedex 5, France.
  • Burel-Vandenbos F; From the Université Nice Sophia Antipolis, CNRS, INSERM, iBV, 06108 Nice, France, the Service d'Anatomopathologie, Hôpital Pasteur, CHU de Nice, Nice 06107, France.
  • Imbert J; INSERM, U1090, Transcriptomic and Genomic Marseille-Luminy/Technical Advances for Genomics and Clinics (TGML/TAGC), Marseille F-13009, France, UMR_S 1090, TGML/TAGC, Aix-Marseille Université, Marseille F-13009, France, jean.imbert@inserm.fr.
  • Virolle T; From the Université Nice Sophia Antipolis, CNRS, INSERM, iBV, 06108 Nice, France, virolle@unice.fr.
J Biol Chem ; 291(20): 10684-99, 2016 May 13.
Article en En | MEDLINE | ID: mdl-27002148
Glioblastomas are the most common primary brain tumors, highly vascularized, infiltrating, and resistant to current therapies. This cancer leads to a fatal outcome in less than 18 months. The aggressive behavior of glioblastomas, including resistance to current treatments and tumor recurrence, has been attributed to glioma stemlike/progenitor cells. The transcription factor EGR1 (early growth response 1), a member of a zinc finger transcription factor family, has been described as tumor suppressor in gliomas when ectopically overexpressed. Although EGR1 expression in human glioblastomas has been associated with patient survival, its precise location in tumor territories as well as its contribution to glioblastoma progression remain elusive. In the present study, we show that EGR1-expressing cells are more frequent in high grade gliomas where the nuclear expression of EGR1 is restricted to proliferating/progenitor cells. We show in primary cultures of glioma stemlike cells that EGR1 contributes to stemness marker expression and proliferation by orchestrating a PDGFA-dependent growth-stimulatory loop. In addition, we demonstrate that EGR1 acts as a positive regulator of several important genes, including SHH, GLI1, GLI2, and PDGFA, previously linked to the maintenance and proliferation of glioma stemlike cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Neoplásicas / Neoplasias Encefálicas / Factor de Crecimiento Derivado de Plaquetas / Regulación Neoplásica de la Expresión Génica / Glioblastoma / Comunicación Autocrina / Proliferación Celular / Proteína 1 de la Respuesta de Crecimiento Precoz / Proteínas de Neoplasias Tipo de estudio: Risk_factors_studies Límite: Female / Humans / Male Idioma: En Revista: J Biol Chem Año: 2016 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Células Madre Neoplásicas / Neoplasias Encefálicas / Factor de Crecimiento Derivado de Plaquetas / Regulación Neoplásica de la Expresión Génica / Glioblastoma / Comunicación Autocrina / Proliferación Celular / Proteína 1 de la Respuesta de Crecimiento Precoz / Proteínas de Neoplasias Tipo de estudio: Risk_factors_studies Límite: Female / Humans / Male Idioma: En Revista: J Biol Chem Año: 2016 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Estados Unidos