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A three-dimensional cell culture system as an in vitro canine mammary carcinoma model for the expression of connective tissue modulators.
Cardoso, T C; Sakamoto, S S; Stockmann, D; Souza, T F B; Ferreira, H L; Gameiro, R; Vieira, F V; Louzada, M J Q; Andrade, A L; Flores, E F.
Afiliación
  • Cardoso TC; Veterinary Medicine School, Department of Support, Production and Animal Health, University of São Paulo State, Laboratory of Animal Virology and Cell Culture, Araçatuba, Brazil.
  • Sakamoto SS; Veterinary Medicine School, Department of Clinical, Surgery and Animal Reproduction, University of São Paulo State, Veterinary Hospital Section, Araçatuba, Brazil.
  • Stockmann D; Veterinary Medicine School, Department of Clinical, Surgery and Animal Reproduction, University of São Paulo State, Veterinary Hospital Section, Araçatuba, Brazil.
  • Souza TFB; Veterinary Medicine School, Department of Clinical, Surgery and Animal Reproduction, University of São Paulo State, Veterinary Hospital Section, Araçatuba, Brazil.
  • Ferreira HL; Veterinary Medicine School, Department of Clinical, Surgery and Animal Reproduction, University of São Paulo State, Veterinary Hospital Section, Araçatuba, Brazil.
  • Gameiro R; Department of de Veterinary Medicine, Faculdade Zotecnia e Engenharia de Alimentos-USP, Pirassununga, SP, Brazil.
  • Vieira FV; Veterinary Medicine School, Department of Support, Production and Animal Health, University of São Paulo State, Laboratory of Animal Virology and Cell Culture, Araçatuba, Brazil.
  • Louzada MJQ; Veterinary Medicine School, Department of Support, Production and Animal Health, University of São Paulo State, Laboratory of Animal Virology and Cell Culture, Araçatuba, Brazil.
  • Andrade AL; Veterinary Medicine School, Department of Support, Production and Animal Health, University of São Paulo State, Laboratory of Animal Virology and Cell Culture, Araçatuba, Brazil.
  • Flores EF; Veterinary Medicine School, Department of Clinical, Surgery and Animal Reproduction, University of São Paulo State, Veterinary Hospital Section, Araçatuba, Brazil.
Vet Comp Oncol ; 15(2): 582-593, 2017 Jun.
Article en En | MEDLINE | ID: mdl-26991309
In this study, derived complex carcinoma (CC) and simple carcinoma (SC) cell lines were established and cultured under two-dimensional (2D) and three-dimensional (3D) conditions. The 3D was performed in six-well AlgiMatrix™ (LifeTechnologies®, Carlsbad, CA, USA) scaffolds, resulting in spheroids sized 50-125 µm for CC and 175-200 µm for SC. Cell viability was demonstrated up to 14 days for both models. Epidermal growth factor receptor (EGFR) was expressed in CC and SC in both systems. However, higher mRNA and protein levels were observed in SC 2D and 3D systems when compared with CC (P < 0.005). The connective tissue modulators, metalloproteinases-1, -2, -9 and -13 (MMPs), relaxin receptors 1 and 2 (RXR1 and RXR2) and E-cadherin (CDH1) were quantitated. All were upregulated similarly when canine mammary tumour (CMT)-derived cell lines were cultured under 3D AlgiMatrix, except CDH1 that was downregulated (P < 0.005). These results are promising towards the used of 3D system to increase a high throughput in vitro canine tumour model.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Mamarias Animales / Tejido Conectivo / Enfermedades de los Perros / Andamios del Tejido Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Vet Comp Oncol Asunto de la revista: MEDICINA VETERINARIA / NEOPLASIAS Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Mamarias Animales / Tejido Conectivo / Enfermedades de los Perros / Andamios del Tejido Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Vet Comp Oncol Asunto de la revista: MEDICINA VETERINARIA / NEOPLASIAS Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido