Your browser doesn't support javascript.
loading
Detection of Campylobacter in human and animal field samples in Cambodia.
Osbjer, Kristina; Tano, Eva; Chhayheng, Leang; Mac-Kwashie, Akofa Olivia; Fernström, Lise-Lotte; Ellström, Patrik; Sokerya, Seng; Sokheng, Choup; Mom, Veng; Chheng, Kannarath; San, Sorn; Davun, Holl; Boqvist, Sofia; Rautelin, Hilpi; Magnusson, Ulf.
Afiliación
  • Osbjer K; Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
  • Tano E; Department of Medical Sciences, Clinical Microbiology and Infectious Medicine, Uppsala University, Uppsala, Sweden.
  • Chhayheng L; National Institute of Public Health, Phnom Penh, Cambodia.
  • Mac-Kwashie AO; Department of Medical Sciences, Clinical Microbiology, Uppsala University, Uppsala, Sweden.
  • Fernström LL; Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.
  • Ellström P; Department of Medical Sciences, Clinical Microbiology, Uppsala University, Uppsala, Sweden.
  • Sokerya S; Department of Medical Biochemistry and Microbiology, Zoonosis Science Center, Uppsala University, Uppsala, Sweden.
  • Sokheng C; Centre for Livestock and Agriculture Development, Phnom Penh, Cambodia.
  • Mom V; National Institute of Public Health, Phnom Penh, Cambodia.
  • Chheng K; National Institute of Public Health, Phnom Penh, Cambodia.
  • San S; National Institute of Public Health, Phnom Penh, Cambodia.
  • Davun H; National Veterinary Research Institute, Phnom Penh, Cambodia.
  • Boqvist S; National Veterinary Research Institute, Phnom Penh, Cambodia.
  • Rautelin H; Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.
  • Magnusson U; Department of Medical Sciences, Clinical Microbiology, Uppsala University, Uppsala, Sweden.
APMIS ; 124(6): 508-15, 2016 Jun.
Article en En | MEDLINE | ID: mdl-26991032
Campylobacter are zoonotic bacteria and a leading cause of human gastroenteritis worldwide with Campylobacter jejuni and C. coli being the most commonly detected species. The aim of this study was to detect Campylobacter in humans and livestock (chickens, ducks, pigs, cattle, water buffalo, quail, pigeons and geese) in rural households by routine culturing and multiplex PCR in faecal samples frozen before analysis. Of 681 human samples, 82 (12%) tested positive by PCR (C. jejuni in 66 samples and C. coli in 16), but none by routine culture. Children were more commonly Campylobacter positive (19%) than adult males (8%) and females (7%). Of 853 livestock samples, 106 (12%) tested positive by routine culture and 352 (41%) by PCR. Campylobacter jejuni was more frequent in chickens and ducks and C. coli in pigs. In conclusion, Campylobacter proved to be highly prevalent by PCR in children (19%), ducks (24%), chickens (56%) and pigs (72%). Routine culturing was insufficiently sensitive in detecting Campylobacter in field samples frozen before analysis. These findings suggest that PCR should be the preferred diagnostic method for detection of Campylobacter in humans and livestock where timely culture is not feasible.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Campylobacter / Infecciones por Campylobacter / Reacción en Cadena de la Polimerasa / Técnicas Bacteriológicas / Técnicas de Diagnóstico Molecular / Heces Tipo de estudio: Diagnostic_studies / Evaluation_studies / Observational_studies / Prevalence_studies / Risk_factors_studies Límite: Adolescent / Adult / Aged / Aged80 / Animals / Child / Child, preschool / Female / Humans / Infant País/Región como asunto: Asia Idioma: En Revista: APMIS Asunto de la revista: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA / PATOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Dinamarca
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Campylobacter / Infecciones por Campylobacter / Reacción en Cadena de la Polimerasa / Técnicas Bacteriológicas / Técnicas de Diagnóstico Molecular / Heces Tipo de estudio: Diagnostic_studies / Evaluation_studies / Observational_studies / Prevalence_studies / Risk_factors_studies Límite: Adolescent / Adult / Aged / Aged80 / Animals / Child / Child, preschool / Female / Humans / Infant País/Región como asunto: Asia Idioma: En Revista: APMIS Asunto de la revista: ALERGIA E IMUNOLOGIA / MICROBIOLOGIA / PATOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Dinamarca