Breaks in the 45S rDNA Lead to Recombination-Mediated Loss of Repeats.
Cell Rep
; 14(11): 2519-27, 2016 Mar 22.
Article
en En
| MEDLINE
| ID: mdl-26972008
rDNA repeats constitute the most heavily transcribed region in the human genome. Tumors frequently display elevated levels of recombination in rDNA, indicating that the repeats are a liability to the genomic integrity of a cell. However, little is known about how cells deal with DNA double-stranded breaks in rDNA. Using selective endonucleases, we show that human cells are highly sensitive to breaks in 45S but not the 5S rDNA repeats. We find that homologous recombination inhibits repair of breaks in 45S rDNA, and this results in repeat loss. We identify the structural maintenance of chromosomes protein 5 (SMC5) as contributing to recombination-mediated repair of rDNA breaks. Together, our data demonstrate that SMC5-mediated recombination can lead to error-prone repair of 45S rDNA repeats, resulting in their loss and thereby reducing cellular viability.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ARN Ribosómico
Límite:
Humans
Idioma:
En
Revista:
Cell Rep
Año:
2016
Tipo del documento:
Article
Pais de publicación:
Estados Unidos