Evaluation of the RapidHIT™ 200 and RapidHIT GlobalFiler(®) Express kit for fully automated STR genotyping.
Forensic Sci Int Genet
; 23: 1-8, 2016 07.
Article
en En
| MEDLINE
| ID: mdl-26971314
The RapidHIT™ 200 Human Identification System and RapidHIT GlobalFiler(®) Express kit were evaluated and validated for use with single-source reference samples. It was of primary interest to evaluate the system for its efficacy as an expert system and to estimate a first pass success rate, as well as to identify the technical variables impacting that result. While results indicated that this instrument/kit combination can be used to accurately type single-source buccal samples, substantial variability in sensitivity and intra-color balance were observed, as were multiple artifacts, requiring extensive manual editing of the profiles. Artifacts included dye "blobs" and spectral overlap (pull-up) peaks that often originated from relatively low intensity allele peaks. Reduced intra-color balance, in combination with low sensitivity, occasionally resulted in instances of allelic dropout. Overall, 50% of the buccal samples analyzed in this study would have been successfully typed to give full GlobalFiler(®) profiles without the need for manual review and editing.
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Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Marcadores Genéticos
/
Dermatoglifia del ADN
/
Repeticiones de Microsatélite
Tipo de estudio:
Evaluation_studies
/
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Forensic Sci Int Genet
Asunto de la revista:
GENETICA
/
JURISPRUDENCIA
Año:
2016
Tipo del documento:
Article
Pais de publicación:
Países Bajos