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Development of a real-time imaging system for hypoxic cell apoptosis.
Kagiya, Go; Ogawa, Ryohei; Hyodo, Fuminori; Yamashita, Kei; Nakamura, Mizuki; Ishii, Ayumi; Sejimo, Yukihiko; Tominaga, Shintaro; Murata, Masaharu; Tanaka, Yoshikazu; Hatashita, Masanori.
Afiliación
  • Kagiya G; School of Allied Health Sciences, Kitasato University , Sagamihara, Kanagawa, Japan.
  • Ogawa R; Department of Radiological Sciences, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama , Sugitani, Toyama, Japan.
  • Hyodo F; Innovation Center for Medical Redox Navigation, Kyushu University , Higashi-ku, Fukuoka, Japan.
  • Yamashita K; Division of Radiation Oncology, Chiba Cancer Center , Chiba, Chiba, Japan.
  • Nakamura M; Department of Diagnostic Radiology, Kitasato University Hospital , Sagamihara, Kanagawa, Japan.
  • Ishii A; Department of Diagnostic Radiology, Saitama Prefectural Cancer Center , Kitaadachi-gun, Saitama, Japan.
  • Sejimo Y; Graduate School of Medicine, Gunma University , Maebashi, Gunma, Japan.
  • Tominaga S; School of Allied Health Sciences, Kitasato University , Sagamihara, Kanagawa, Japan.
  • Murata M; Innovation Center for Medical Redox Navigation, Kyushu University , Higashi-ku, Fukuoka, Japan.
  • Tanaka Y; Biology Group, The Wakasa Wan Energy Research Center, Nagatani , Tsuruga, Fukui, Japan.
  • Hatashita M; Biology Group, The Wakasa Wan Energy Research Center, Nagatani , Tsuruga, Fukui, Japan.
Mol Ther Methods Clin Dev ; 5: 16009, 2016.
Article en En | MEDLINE | ID: mdl-26966700
Hypoxic regions within the tumor form due to imbalances between cell proliferation and angiogenesis; specifically, temporary closure or a reduced flow due to abnormal vasculature. They create environments where cancer cells acquire resistance to therapies. Therefore, the development of therapeutic approaches targeting the hypoxic cells is one of the most crucial challenges for cancer regression. Screening potential candidates for effective diagnostic modalities even under a hypoxic environment would be an important first step. In this study, we describe the development of a real-time imaging system to monitor hypoxic cell apoptosis for such screening. The imaging system is composed of a cyclic luciferase (luc) gene under the control of an improved hypoxic-responsive promoter. The cyclic luc gene product works as a caspase-3 (cas-3) monitor as it gains luc activity in response to cas-3 activation. The promoter composed of six hypoxic responsible elements and the CMV IE1 core promoter drives the effective expression of the cyclic luc gene in hypoxic conditions, enhancing hypoxic cell apoptosis visualization. We also confirmed real-time imaging of hypoxic cell apoptosis in the spheroid, which shares properties with the tumor. Thus, this constructed system could be a powerful tool for the development of effective anticancer diagnostic modalities.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Mol Ther Methods Clin Dev Año: 2016 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Mol Ther Methods Clin Dev Año: 2016 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos