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Mechanical oscillations enhance gene delivery into suspended cells.
Zhou, Z L; Sun, X X; Ma, J; Man, C H; Wong, A S T; Leung, A Y; Ngan, A H W.
Afiliación
  • Zhou ZL; Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, P.R. China.
  • Sun XX; Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, P.R. China.
  • Ma J; School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, P.R. China.
  • Man CH; Department of Medicine, The University of Hong Kong, Pokfulam Road, Hong Kong, P.R. China.
  • Wong AS; School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong, P.R. China.
  • Leung AY; Department of Medicine, The University of Hong Kong, Pokfulam Road, Hong Kong, P.R. China.
  • Ngan AH; Department of Mechanical Engineering, The University of Hong Kong, Pokfulam Road, Hong Kong, P.R. China.
Sci Rep ; 6: 22824, 2016 Mar 09.
Article en En | MEDLINE | ID: mdl-26956215
Suspended cells are difficult to be transfected by common biochemical methods which require cell attachment to a substrate. Mechanical oscillations of suspended cells at certain frequencies are found to result in significant increase in membrane permeability and potency for delivery of nano-particles and genetic materials into the cells. Nanomaterials including siRNAs are found to penetrate into suspended cells after subjecting to short-time mechanical oscillations, which would otherwise not affect the viability of the cells. Theoretical analysis indicates significant deformation of the actin-filament network in the cytoskeleton cortex during mechanical oscillations at the experimental frequency, which is likely to rupture the soft phospholipid bilayer leading to increased membrane permeability. The results here indicate a new method for enhancing cell transfection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Mecánico / Transformación Genética / Transfección Límite: Humans Idioma: En Revista: Sci Rep Año: 2016 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Estrés Mecánico / Transformación Genética / Transfección Límite: Humans Idioma: En Revista: Sci Rep Año: 2016 Tipo del documento: Article Pais de publicación: Reino Unido