[EFFECT OF PULSE-PERIODIC CORONA DISCHARGE ON VIABILITY OF ESCHERICHIA COLI M17 CELLS IN BIOFILMS].
Zh Mikrobiol Epidemiol Immunobiol
; (6): 17-23, 2015.
Article
en Ru
| MEDLINE
| ID: mdl-26950984
AIM: Detection of bactericidal effect of pulse-periodic corona discharge (PPCD) on cells and biofilms of Escherichia coli M17. MATERIALS AND METHODS: A gas-discharge device was created based on PPCD in air with power supply parameters: amplitude values of voltage of 30 - 60 kV, pulse repetition rate of 250 - 400 kHz. Ultrastructure changes in cells and biofilms of E. coli M17, affected by PPCD, generated in air, were studied by typical methods of transmission electron microscopy. RESULTS: Disturbances of integrity of surface and abyssal structures of biofilms, as well as changes of morphological properties of E. coli M17 cells, characteristic for sub-lethal heat impact, were detected. Destructive changes of bacterial cells were developed by formation of focal disturbance of cytoplasmic membrane, extension of periplasmic space, formation of globular structures, characteristic for heat effect, and destruction of cytoplasm. CONCLUSION: Bactericidal effect of PPCD on E. coli M17 cells as part of biofilms was shown. Destructive morphological changes in cells and biofilms of E. coli M17 after the effect of PPCD were detected for the first time on electron-microscopic level.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Membrana Celular
/
Biopelículas
/
Periplasma
/
Escherichia coli
/
Gases em Plasma
Idioma:
Ru
Revista:
Zh Mikrobiol Epidemiol Immunobiol
Año:
2015
Tipo del documento:
Article
Pais de publicación:
Rusia