Preparation and evaluation of dual-enzyme microreactor with co-immobilized trypsin and chymotrypsin.

Meller, Kinga; Pomastowski, Pawel; Grzywinski, Damian; Szumski, Michal; Buszewski, Boguslaw

Meller, Kinga; Pomastowski, Pawel; Grzywinski, Damian; Szumski, Michal; Buszewski, Boguslaw.

Afiliación

Meller K; Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarina 7, 87-100 Torun, Poland.
Pomastowski P; Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarina 7, 87-100 Torun, Poland; Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University in Torun, Wilenska 4, 87-100 Torun, Poland.
Grzywinski D; Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarina 7, 87-100 Torun, Poland; Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University in Torun, Wilenska 4, 87-100 Torun, Poland.
Szumski M; Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarina 7, 87-100 Torun, Poland; Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University in Torun, Wilenska 4, 87-100 Torun, Poland. Electronic address: michu@ch
Buszewski B; Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University in Torun, Gagarina 7, 87-100 Torun, Poland; Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University in Torun, Wilenska 4, 87-100 Torun, Poland.

J Chromatogr A ; 1440: 45-54, 2016 Apr 01.

Article en En | MEDLINE | ID: mdl-26947160

Asunto(s)

Quimotripsina/química; Enzimas Inmovilizadas/metabolismo; Proteómica/métodos; Tripsina/química; Arginina/análogos & derivados; Arginina/química; Reactores Biológicos; Cromatografía Liquida; Compuestos Epoxi/química; Humanos; Metacrilatos/química; Compuestos de Organosilicio/química; Polimerizacion; Proteínas/metabolismo; Silanos/química; Dióxido de Silicio/química

Palabras clave

Capillary liquid chromatography; Chymotrypsin; Immobilized enzyme microreactor; Proteomics; Transferrin; Trypsin

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Quimotripsina / Tripsina / Proteómica / Enzimas Inmovilizadas Límite: Humans Idioma: En Revista: J Chromatogr A Año: 2016 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Países Bajos

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