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In vivo immunomodulatory effects of adipose-derived mesenchymal stem cells conditioned medium in experimental autoimmune encephalomyelitis.
Yousefi, Forouzan; Ebtekar, Massoumeh; Soudi, Sara; Soleimani, Masoud; Hashemi, Seyed Mahmoud.
Afiliación
  • Yousefi F; Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
  • Ebtekar M; Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address: ebtekarm@modares.ac.ir.
  • Soudi S; Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
  • Soleimani M; Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
  • Hashemi SM; Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: smmhashemi@sbmu.ac.ir.
Immunol Lett ; 172: 94-105, 2016 04.
Article en En | MEDLINE | ID: mdl-26930038
Mesenchymal stem cells (MSCs) are well known to possess neuroprotective and immunomodulatory effects, due to cell-to-cell interaction and their soluble factors. We conducted a comparative analysis of the immunomodulatory properties of adipose tissue mesenchymal stem cells (AT-MSCs) and their conditioned media (CM), derived from C57/BL6 mice, for mitigating the adverse clinical course of experimental autoimmune encephalomyelitis (EAE). We measure IL4, IL17 and IFNÉ£ production of supernatant from spleen cells. We analyzed brain cell infiltration, splenocyte proliferation and evaluated the percentage of CD4+CD25+FOXP3+splenic cell population in all EAE C57/BL6 mice. AT-MSCs and its conditioned medium induced CD4+CD25+FOXP3+regulatory T cells after in vitro co-culture with naïve T cells. There is no significant difference in the clinical scores and body weight of EAE mice treated with AT-MSCs and CM. The reduction in proliferative responses and brain cell infiltration was more pronounced in mice injected with CM than other groups. It is found that the percentage of splenic CD4+CD25+FOXP3+ population as well as the level of IL4 production in mice administrated with AT-MSCs is increased compared to other animals. Our results suggest that AT-MSCs-derived CM is promising in stem cell therapy, due to their neuroprotective and immunomudulatory properties.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Encéfalo / Tejido Adiposo / Linfocitos T Reguladores / Encefalomielitis Autoinmune Experimental / Células Madre Mesenquimatosas / Esclerosis Múltiple Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Immunol Lett Año: 2016 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Encéfalo / Tejido Adiposo / Linfocitos T Reguladores / Encefalomielitis Autoinmune Experimental / Células Madre Mesenquimatosas / Esclerosis Múltiple Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Immunol Lett Año: 2016 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Países Bajos