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Silkomics: Insight into the Silk Spinning Process of Spiders.
Dos Santos-Pinto, José Roberto Aparecido; Garcia, Ana Maria Caviquioli; Arcuri, Helen Andrade; Esteves, Franciele Grego; Salles, Heliana Clara; Lubec, Gert; Palma, Mario Sergio.
Afiliación
  • Dos Santos-Pinto JR; Center of Study of Social Insects, Department of Biology, Institute of Biosciences of Rio Claro, São Paulo State University (UNESP) , Rio Claro, São Paulo 13500, Brazil.
  • Garcia AM; Department of Pediatrics, Medical University of Vienna , Vienna 1090, Austria.
  • Arcuri HA; Center of Study of Social Insects, Department of Biology, Institute of Biosciences of Rio Claro, São Paulo State University (UNESP) , Rio Claro, São Paulo 13500, Brazil.
  • Esteves FG; Center of Study of Social Insects, Department of Biology, Institute of Biosciences of Rio Claro, São Paulo State University (UNESP) , Rio Claro, São Paulo 13500, Brazil.
  • Salles HC; Center of Study of Social Insects, Department of Biology, Institute of Biosciences of Rio Claro, São Paulo State University (UNESP) , Rio Claro, São Paulo 13500, Brazil.
  • Lubec G; Center of Study of Social Insects, Department of Biology, Institute of Biosciences of Rio Claro, São Paulo State University (UNESP) , Rio Claro, São Paulo 13500, Brazil.
  • Palma MS; Department of Pediatrics, Medical University of Vienna , Vienna 1090, Austria.
J Proteome Res ; 15(4): 1179-93, 2016 Apr 01.
Article en En | MEDLINE | ID: mdl-26923066
The proteins from the silk-producing glands were identified using both a bottom-up gel-based proteomic approach as well as from a shotgun proteomic approach. Additionally, the relationship between the functions of identified proteins and the spinning process was studied. A total of 125 proteins were identified in the major ampullate, 101 in the flagelliform, 77 in the aggregate, 75 in the tubuliform, 68 in the minor ampullate, and 23 in aciniform glands. On the basis of the functional classification using Gene Ontology, these proteins were organized into seven different groups according to their general function: (i) web silk proteins-spidroins, (ii) proteins related to the folding/conformation of spidroins, (iii) proteins that protect silk proteins from oxidative stress, (iv) proteins involved in fibrillar preservation of silks in the web, (v) proteins related to ion transport into and out of the glands during silk fiber spinning, (vi) proteins involved in prey capture and pre-digestion, and (vii) housekeeping proteins from all of the glands. Thus, a general mechanism of action for the identified proteins in the silk-producing glands from the Nephila clavipes spider was proposed; the current results also indicate that the webs play an active role in prey capture.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arañas / Proteínas de Insectos / Estructuras Animales / Proteómica / Seda Límite: Animals Idioma: En Revista: J Proteome Res Asunto de la revista: BIOQUIMICA Año: 2016 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arañas / Proteínas de Insectos / Estructuras Animales / Proteómica / Seda Límite: Animals Idioma: En Revista: J Proteome Res Asunto de la revista: BIOQUIMICA Año: 2016 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos