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Identifying a Carotenoid Cleavage Dioxygenase 4a Gene and Its Efficient Agrobacterium-Mediated Genetic Transformation in Bixa orellana L.
Sankari, Mohan; Hemachandran, Hridya; Anantharaman, Amirtha; Babu, Subramanian; Madrid, Renata Rivera; C, George Priya Doss; Fulzele, Devanand P; Siva, Ramamoorthy.
Afiliación
  • Sankari M; School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.
  • Hemachandran H; School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.
  • Anantharaman A; School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.
  • Babu S; School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.
  • Madrid RR; Cenro de Investigacion Cientifica de Yucatan, A.C. Calle 43 No. 130, Col. Chuburnade Hidalgo, 97200, Merida, Yucatan, Mexico.
  • C GP; School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India.
  • Fulzele DP; Plant Biotechnology and Secondary Metabolites Section, NA&BTD, Bhabha Atomic Research Centre, Mumbai, Maharashtra, India.
  • Siva R; School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India. rsiva77in@rediffmail.com.
Appl Biochem Biotechnol ; 179(5): 697-714, 2016 Jul.
Article en En | MEDLINE | ID: mdl-26922728
Carotenoids are metabolized to apocarotenoids through the pathway catalysed by carotenoid cleavage oxygenases (CCOs). The apocarotenoids are economically important as it is known to have therapeutic as well as industrial applications. For instance, bixin from Bixa orellana and crocin from Crocus sativus are commercially used as a food colourant and cosmetics since prehistoric time. In our present study, CCD4a gene has been identified and isolated from leaves of B. orellana for the first time and named as BoCCD4a; phylogenetic analysis was carried out using CLUSTAL W. From sequence analysis, BoCCD4a contains two exons and one intron, which was compared with the selected AtCCD4, RdCCD4, GmCCD4 and CmCCD4a gene. Further, the BoCCD4a gene was cloned into pCAMBIA 1301, transformed into Agrobacterium tumefaciens EHA105 strain and subsequently transferred into hypocotyledons and callus of B. orellana by agro-infection. Selection of stable transformation was screened on the basis of PCR detection by using GUS and hptII specific primer, which was followed by histochemical characterization. The percent transient GUS expression in hypocotyledons and callus was 84.4 and 80 %, respectively. The expression of BoCCD4a gene in B. orellana was confirmed through RT-PCR analysis. From our results, the sequence analysis of BoCCD4a gene of B. orellana was closely related to the CsCCD4 gene of C. sativus, which suggests this gene may have a role in various processes such as fragrance, insect attractant and pollination.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxigenasas / Filogenia / Carotenoides / Proteínas de Arabidopsis / Dioxigenasas Idioma: En Revista: Appl Biochem Biotechnol Año: 2016 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxigenasas / Filogenia / Carotenoides / Proteínas de Arabidopsis / Dioxigenasas Idioma: En Revista: Appl Biochem Biotechnol Año: 2016 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos