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Production of Recombinant Chemokines and Validation of Refolding.
Veldkamp, Christopher T; Koplinski, Chad A; Jensen, Davin R; Peterson, Francis C; Smits, Kaitlin M; Smith, Brittney L; Johnson, Scott K; Lettieri, Christina; Buchholz, Wallace G; Solheim, Joyce C; Volkman, Brian F.
Afiliación
  • Veldkamp CT; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin, USA; Department of Chemistry, University of Wisconsin-Whitewater, Whitewater, Wisconsin, USA. Electronic address: veldkamc@uww.edu.
  • Koplinski CA; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin, USA.
  • Jensen DR; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin, USA.
  • Peterson FC; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin, USA.
  • Smits KM; Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska, USA; Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA; The Eppley Institute and the Fred and Pamela Buffett Cancer Center, University of Nebr
  • Smith BL; Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska, USA; Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA; The Eppley Institute and the Fred and Pamela Buffett Cancer Center, University of Nebr
  • Johnson SK; Biological Process Development Facility, College of Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.
  • Lettieri C; Department of Pediatrics, Children's Hospital and Medical Center, University of Nebraska Medical Center, Omaha, Nebraska, USA.
  • Buchholz WG; Biological Process Development Facility, College of Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.
  • Solheim JC; Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska, USA; Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA; The Eppley Institute and the Fred and Pamela Buffett Cancer Center, University of Nebr
  • Volkman BF; Department of Biochemistry, Medical College of Wisconsin, Milwaukee, Wisconsin, USA.
Methods Enzymol ; 570: 539-65, 2016.
Article en En | MEDLINE | ID: mdl-26921961
The diverse roles of chemokines in normal immune function and many human diseases have motivated numerous investigations into the structure and function of this family of proteins. Recombinant chemokines are often used to study how chemokines coordinate the trafficking of immune cells in various biological contexts. A reliable source of biologically active protein is vital for any in vitro or in vivo functional analysis. In this chapter, we describe a general method for the production of recombinant chemokines and robust techniques for efficient refolding that ensure consistently high biological activity. Considerations for initiating development of protocols consistent with Current Good Manufacturing Practices (cGMPs) to produce biologically active chemokines suitable for use in clinical trials are also discussed.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Recombinantes / Ingeniería de Proteínas Tipo de estudio: Guideline Idioma: En Revista: Methods Enzymol Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Recombinantes / Ingeniería de Proteínas Tipo de estudio: Guideline Idioma: En Revista: Methods Enzymol Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos