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Cytotoxic effect of Erythroxylum daphnites extract is associated with G1 cell cycle arrest and apoptosis in oral squamous cell carcinoma.
Elias, Silvia T; Macedo, Carolina C S; Simeoni, Luiz A; Silveira, Dâmaris; Magalhães, Pérola O; Lofrano-Porto, Adriana; Coletta, Ricardo D; Neves, Francisco A R; Guerra, Eliete N S.
Afiliación
  • Elias ST; a Faculty of Health Sciences, University of Brasília , Brasília , Brazil.
  • Macedo CC; b Faculty of Dentistry, University of Campinas , Piracicaba , São Paulo , Brazil.
  • Simeoni LA; a Faculty of Health Sciences, University of Brasília , Brasília , Brazil.
  • Silveira D; a Faculty of Health Sciences, University of Brasília , Brasília , Brazil.
  • Magalhães PO; a Faculty of Health Sciences, University of Brasília , Brasília , Brazil.
  • Lofrano-Porto A; a Faculty of Health Sciences, University of Brasília , Brasília , Brazil.
  • Coletta RD; b Faculty of Dentistry, University of Campinas , Piracicaba , São Paulo , Brazil.
  • Neves FA; a Faculty of Health Sciences, University of Brasília , Brasília , Brazil.
  • Guerra EN; a Faculty of Health Sciences, University of Brasília , Brasília , Brazil.
Cell Cycle ; 15(7): 948-56, 2016.
Article en En | MEDLINE | ID: mdl-26918580
Plant-derived molecules showing antineoplastic effects have recently gained increased attention as potential adjuvants to traditional therapies for various cancers. Cerrado biome in Brazil contains high floral biodiversity, but knowledge about the potential therapeutic effects of compounds derived from that flora is still limited. The present study investigated the antineoplastic activity of Erythroxylum daphnites Mart., a Brazilian native plant from Cerrado biome, in the SCC-9 oral squamous cell carcinoma cell line. Cells were treated with various concentrations of hexane extract of Erythroxylum daphnites leaves (EDH) and assessed for cytotoxicity, proliferation, and apoptosis. Thin layer chromatography was conducted to characterize the substances present in EDH. Our results showed that EDH exerted anti-proliferative effects in SCC-9 cells by stabilizing the cell cycle at G1 phase in association with reduced intracellular levels of cyclins D and E and increased level of p21. EDH also demonstrated pro-apoptotic properties, as shown by an increased expression of caspase-3. Triterpenes were the major constituents of EDH. Our findings demonstrated a cytotoxic effect of EDH against SCC-9 cells in vitro mediated by the restraint of cellular proliferation and induction of apoptosis. Taken together, these findings support EDH constituents as potential therapeutic adjuvants for oral cancer.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Boca / Carcinoma de Células Escamosas / Apoptosis / Erythroxylaceae / Puntos de Control de la Fase G1 del Ciclo Celular / Antineoplásicos Fitogénicos Tipo de estudio: Risk_factors_studies Límite: Humans Idioma: En Revista: Cell Cycle Año: 2016 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Boca / Carcinoma de Células Escamosas / Apoptosis / Erythroxylaceae / Puntos de Control de la Fase G1 del Ciclo Celular / Antineoplásicos Fitogénicos Tipo de estudio: Risk_factors_studies Límite: Humans Idioma: En Revista: Cell Cycle Año: 2016 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Estados Unidos