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Polyethersulfone improves isothermal nucleic acid amplification compared to current paper-based diagnostics.
Linnes, J C; Rodriguez, N M; Liu, L; Klapperich, C M.
Afiliación
  • Linnes JC; Department of Biomedical Engineering, Boston University, Boston, MA, 02215, USA.
  • Rodriguez NM; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, 47907, USA.
  • Liu L; Department of Biomedical Engineering, Boston University, Boston, MA, 02215, USA.
  • Klapperich CM; Department of Biomedical Engineering, Boston University, Boston, MA, 02215, USA.
Biomed Microdevices ; 18(2): 30, 2016 Apr.
Article en En | MEDLINE | ID: mdl-26906904
Devices based on rapid, paper-based, isothermal nucleic acid amplification techniques have recently emerged with the potential to fill a growing need for highly sensitive point-of-care diagnostics throughout the world. As this field develops, such devices will require optimized materials that promote amplification and sample preparation. Herein, we systematically investigated isothermal nucleic acid amplification in materials currently used in rapid diagnostics (cellulose paper, glass fiber, and nitrocellulose) and two additional porous membranes with upstream sample preparation capabilities (polyethersulfone and polycarbonate). We compared amplification efficiency from four separate DNA and RNA targets (Bordetella pertussis, Chlamydia trachomatis, Neisseria gonorrhoeae, and Influenza A H1N1) within these materials using two different isothermal amplification schemes, helicase dependent amplification (tHDA) and loop-mediated isothermal amplification (LAMP), and traditional PCR. We found that the current paper-based diagnostic membranes inhibited nucleic acid amplification when compared to membrane-free controls; however, polyethersulfone allowed for efficient amplification in both LAMP and tHDA reactions. Further, observing the performance of traditional PCR amplification within these membranes was not predicative of their effects on in situ LAMP and tHDA. Polyethersulfone is a new material for paper-based nucleic acid amplification, yet provides an optimal support for rapid molecular diagnostics for point-of-care applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Papel / Polímeros / Sulfonas / Sistemas de Atención de Punto / Técnicas y Procedimientos Diagnósticos / Técnicas de Amplificación de Ácido Nucleico Tipo de estudio: Diagnostic_studies Idioma: En Revista: Biomed Microdevices Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Papel / Polímeros / Sulfonas / Sistemas de Atención de Punto / Técnicas y Procedimientos Diagnósticos / Técnicas de Amplificación de Ácido Nucleico Tipo de estudio: Diagnostic_studies Idioma: En Revista: Biomed Microdevices Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos