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Successful slush nitrogen vitrification of human ovarian tissue.
Talevi, Riccardo; Barbato, Vincenza; Fiorentino, Ilaria; Braun, Sabrina; De Stefano, Cristofaro; Ferraro, Raffaele; Sudhakaran, Sam; Gualtieri, Roberto.
Afiliación
  • Talevi R; Dipartimento di Biologia, Università di Napoli "Federico II", Complesso Universitario di Monte S Angelo, Napoli, Italy. Electronic address: riccardo.talevi@unina.it.
  • Barbato V; Dipartimento di Biologia, Università di Napoli "Federico II", Complesso Universitario di Monte S Angelo, Napoli, Italy.
  • Fiorentino I; Dipartimento di Biologia, Università di Napoli "Federico II", Complesso Universitario di Monte S Angelo, Napoli, Italy.
  • Braun S; Dipartimento di Biologia, Università di Napoli "Federico II", Complesso Universitario di Monte S Angelo, Napoli, Italy.
  • De Stefano C; Unità di Fisiopatologia della Riproduzione e Sterilità di Coppia, Azienda Ospedaliera San Giuseppe Moscati, Avellino, Italy.
  • Ferraro R; Iatropolis, Genesis Day Surgery Sterilità della Coppia, Caserta, Italy.
  • Sudhakaran S; Dipartimento di Biologia, Università di Napoli "Federico II", Complesso Universitario di Monte S Angelo, Napoli, Italy.
  • Gualtieri R; Dipartimento di Biologia, Università di Napoli "Federico II", Complesso Universitario di Monte S Angelo, Napoli, Italy.
Fertil Steril ; 105(6): 1523-1531.e1, 2016 06.
Article en En | MEDLINE | ID: mdl-26902858
OBJECTIVE: To study whether slush nitrogen vitrification improves the preservation of human ovarian tissue. DESIGN: Control vs. treatment study. SETTING: University research laboratory. PATIENT(S): Ovarian biopsies collected from nine women (aged 14-35 years) during laparoscopic surgery for benign gynecologic conditions. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Ovarian cortical strips of 2 × 5 × 1 mm were vitrified with liquid or slush nitrogen. Fresh and vitrified cortical strips were analyzed for cryodamage and viability under light, confocal, and transmission electron microscopy. RESULT(S): Compared with liquid nitrogen, vitrification with slush nitrogen preserves [1] follicle quality (grade 1 follicles: fresh control, 50%; liquid nitrogen, 27%; slush nitrogen, 48%); [2] granulosa cell ultrastructure (intact cells: fresh control, 92%; liquid nitrogen, 45%; slush nitrogen, 73%), stromal cell ultrastructure (intact cells: fresh control, 59.8%; liquid nitrogen, 24%; slush nitrogen, 48.7%), and DNA integrity (TUNEL-positive cells: fresh control, 0.5%; liquid nitrogen, 2.3%; slush nitrogen, 0.4%); and [3] oocyte, granulosa, and stromal cell viability (oocyte: fresh control, 90%; liquid nitrogen, 63%; slush nitrogen, 87%; granulosa cells: fresh control, 93%; liquid nitrogen, 53%; slush nitrogen, 81%; stromal cells: fresh control, 63%; liquid nitrogen, 30%; slush nitrogen, 52%). CONCLUSION(S): The histology, ultrastructure, and viability of follicles and stromal cells are better preserved after vitrification with slush nitrogen compared with liquid nitrogen.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Criopreservación / Crioprotectores / Vitrificación / Folículo Ovárico / Nitrógeno Límite: Adolescent / Adult / Female / Humans Idioma: En Revista: Fertil Steril Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Criopreservación / Crioprotectores / Vitrificación / Folículo Ovárico / Nitrógeno Límite: Adolescent / Adult / Female / Humans Idioma: En Revista: Fertil Steril Año: 2016 Tipo del documento: Article Pais de publicación: Estados Unidos