The sunflower transcription factor HaHB11 improves yield, biomass and tolerance to flooding in transgenic Arabidopsis plants.

Cabello, Julieta V; Giacomelli, Jorge I; Piattoni, Claudia V; Iglesias, Alberto A; Chan, Raquel L

Cabello, Julieta V; Giacomelli, Jorge I; Piattoni, Claudia V; Iglesias, Alberto A; Chan, Raquel L.

Afiliación

Cabello JV; Instituto de Agrobiotecnología del Litoral, CONICET, Universidad Nacional del Litoral, Colectora Ruta Nacional 168 km 0, 3000 Santa Fe, Argentina.
Giacomelli JI; Instituto de Agrobiotecnología del Litoral, CONICET, Universidad Nacional del Litoral, Colectora Ruta Nacional 168 km 0, 3000 Santa Fe, Argentina.
Piattoni CV; Instituto de Agrobiotecnología del Litoral, CONICET, Universidad Nacional del Litoral, Colectora Ruta Nacional 168 km 0, 3000 Santa Fe, Argentina.
Iglesias AA; Instituto de Agrobiotecnología del Litoral, CONICET, Universidad Nacional del Litoral, Colectora Ruta Nacional 168 km 0, 3000 Santa Fe, Argentina.
Chan RL; Instituto de Agrobiotecnología del Litoral, CONICET, Universidad Nacional del Litoral, Colectora Ruta Nacional 168 km 0, 3000 Santa Fe, Argentina. Electronic address: rchan@fbcb.unl.edu.ar.

J Biotechnol ; 222: 73-83, 2016 Mar 20.

Article en En | MEDLINE | ID: mdl-26876611

RESUMEN

HaHB11 is a member of the sunflower homeodomain-leucine zipper I subfamily of transcription factors. The analysis of a sunflower microarray hybridized with RNA from HaHB11-transformed leaf-disks indicated the regulation of many genes encoding enzymes from glycolisis and fermentative pathways. A 1300bp promoter sequence, fused to the GUS reporter gene, was used to transform Arabidopsis plants showing an induction of expression after flooding treatments, concurrently with HaHB11 regulation by submergence in sunflower. Arabidopsis transgenic plants expressing HaHB11 under the control of the CaMV 35S promoter and its own promoter were obtained and these plants exhibited significant increases in rosette and stem biomass. All the lines produced more seeds than controls and particularly, those of high expression level doubled seeds yield. Transgenic plants also showed tolerance to flooding stress, both to submergence and waterlogging. Carbohydrates contents were higher in the transgenics compared to wild type and decreased less after submergence treatments. Finally, transcript levels of selected genes involved in glycolisis and fermentative pathways as well as the corresponding enzymatic activities were assessed both, in sunflower and transgenic Arabidopsis plants, before and after submergence. Altogether, the present work leads us to propose HaHB11 as a biotechnological tool to improve crops yield, biomass and flooding tolerance.

Asunto(s)

Arabidopsis/fisiología; Helianthus/genética; Proteínas de Plantas/metabolismo; Plantas Modificadas Genéticamente/fisiología; Proteínas Recombinantes/metabolismo; Factores de Transcripción/metabolismo; Adaptación Biológica/genética; Arabidopsis/genética; Arabidopsis/metabolismo; Biomasa; Inundaciones; Proteínas de Homeodominio/química; Proteínas de Homeodominio/genética; Proteínas de Homeodominio/metabolismo; Leucina Zippers/genética; Proteínas de Plantas/química; Proteínas de Plantas/genética; Plantas Modificadas Genéticamente/genética; Plantas Modificadas Genéticamente/metabolismo; Proteínas Recombinantes/química; Proteínas Recombinantes/genética; Factores de Transcripción/química; Factores de Transcripción/genética

Palabras clave

Arabidopsis; Flooding; HaHB11; Homeodomain-leucine zipper; Waterlogging; Yield improvement

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Factores de Transcripción / Proteínas Recombinantes / Plantas Modificadas Genéticamente / Arabidopsis / Helianthus Idioma: En Revista: J Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Argentina Pais de publicación: Países Bajos

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