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Human bronchial epithelial cells exposed in vitro to diesel exhaust particles exhibit alterations in cell rheology and cytotoxicity associated with decrease in antioxidant defenses and imbalance in pro- and anti-apoptotic gene expression.
Seriani, Robson; de Souza, Claudia Emanuele Carvalho; Krempel, Paloma Gava; Frias, Daniela Perroni; Matsuda, Monique; Correia, Aristides Tadeu; Ferreira, Márcia Zotti Justo; Alencar, Adriano Mesquita; Negri, Elnara Marcia; Saldiva, Paulo Hilário Nascimento; Mauad, Thais; Macchione, Mariangela.
Afiliación
  • Seriani R; Laboratory of Experimental Air Pollution (LIM05), Department of Pathology, School of Medicine, University of São Paulo, Av. Dr. Arnaldo 455, 1°andar, sala 1150, Cerqueira César, São Paulo, SP, CEP:01246-903, Brazil. robsonseriani@yahoo.com.br.
  • de Souza CE; FAM - Faculdades das Américas, Rua Augusta, 1508, 3°andar, São Paulo, SP, 01304-001, Brazil. robsonseriani@yahoo.com.br.
  • Krempel PG; Laboratory of Chronopharmacology, Department of Physiology, Institute of Bioscience, University of São Paulo, Rua do Matão - travessa 14, Cidade Universitária, 05508900, São Paulo, SP, Brazil.
  • Frias DP; Laboratory for Investigations in Ophthalmology (LIM-33), University of São Paulo Medical School São Paulo, Dr. Arnaldo 455, 5°andar, Cerqueira César, São Paulo, SP, CEP:01246-903, Brazil.
  • Matsuda M; Laboratory of Experimental Air Pollution (LIM05), Department of Pathology, School of Medicine, University of São Paulo, Av. Dr. Arnaldo 455, 1°andar, sala 1150, Cerqueira César, São Paulo, SP, CEP:01246-903, Brazil.
  • Correia AT; Laboratory for Investigations in Ophthalmology (LIM-33), University of São Paulo Medical School São Paulo, Dr. Arnaldo 455, 5°andar, Cerqueira César, São Paulo, SP, CEP:01246-903, Brazil.
  • Ferreira MZ; Heart Institute (InCor), Department of Cardiopulmonology, University of São Paulo Medical School, Av. Dr. Enéas de Carvalho Aguiar, 44, Cerqueira Cesar, 05403-000, Sao Paulo, SP, Brazil.
  • Alencar AM; Laboratory of Microrheology and Molecular Physiology, Institute of Physics, University of São Paulo, Rua do Matão, Travessa R Número 187, Cidade Universitária, 05508-090, Sao Paulo, SP, Brazil.
  • Negri EM; Laboratory of Microrheology and Molecular Physiology, Institute of Physics, University of São Paulo, Rua do Matão, Travessa R Número 187, Cidade Universitária, 05508-090, Sao Paulo, SP, Brazil.
  • Saldiva PH; Laboratory of Experimental Air Pollution (LIM05), Department of Pathology, School of Medicine, University of São Paulo, Av. Dr. Arnaldo 455, 1°andar, sala 1150, Cerqueira César, São Paulo, SP, CEP:01246-903, Brazil.
  • Mauad T; Laboratory of Experimental Air Pollution (LIM05), Department of Pathology, School of Medicine, University of São Paulo, Av. Dr. Arnaldo 455, 1°andar, sala 1150, Cerqueira César, São Paulo, SP, CEP:01246-903, Brazil.
  • Macchione M; Laboratory of Experimental Air Pollution (LIM05), Department of Pathology, School of Medicine, University of São Paulo, Av. Dr. Arnaldo 455, 1°andar, sala 1150, Cerqueira César, São Paulo, SP, CEP:01246-903, Brazil.
Environ Sci Pollut Res Int ; 23(10): 9862-70, 2016 May.
Article en En | MEDLINE | ID: mdl-26856867
Diesel exhaust particles (DEPs) from diesel engines produce adverse alterations in cells of the airways by activating intracellular signaling pathways and apoptotic gene overexpression, and also by influencing metabolism and cytoskeleton changes. This study used human bronchial epithelium cells (BEAS-2B) in culture and evaluates their exposure to DEPs (15ug/mL for 1 and 2 h) in order to determine changes to cell rheology (viscoelasticity) and gene expression of the enzymes involved in oxidative stress, apoptosis, and cytotoxicity. BEAS-2B cells exposed to DEPs were found to have a significant loss in stiffness, membrane stability, and mitochondrial activity. The genes involved in apoptosis [B cell lymphoma 2 (BCL-2 and caspase-3)] presented inversely proportional expressions (p = 0.05, p = 0.01, respectively), low expression of the genes involved in antioxidant responses [SOD1 (superoxide dismutase 1); SOD2 (superoxide dismutase 2), and GPx (glutathione peroxidase) (p = 0.01)], along with an increase in cytochrome P450, family 1, subfamily A, polypeptide 1 (CYP1A1) (p = 0.01). These results suggest that alterations in cell rheology and cytotoxicity could be associated with oxidative stress and imbalance between pro- and anti-apoptotic genes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Emisiones de Vehículos / Bronquios / Expresión Génica / Apoptosis / Células Epiteliales / Material Particulado / Antioxidantes Tipo de estudio: Risk_factors_studies Límite: Humans Idioma: En Revista: Environ Sci Pollut Res Int Asunto de la revista: SAUDE AMBIENTAL / TOXICOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Emisiones de Vehículos / Bronquios / Expresión Génica / Apoptosis / Células Epiteliales / Material Particulado / Antioxidantes Tipo de estudio: Risk_factors_studies Límite: Humans Idioma: En Revista: Environ Sci Pollut Res Int Asunto de la revista: SAUDE AMBIENTAL / TOXICOLOGIA Año: 2016 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Alemania