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Mass spectrometry characterization of circulating human serum albumin microheterogeneity in patients with alcoholic hepatitis.
Naldi, Marina; Baldassarre, Maurizio; Domenicali, Marco; Giannone, Ferdinando Antonino; Bossi, Matteo; Montomoli, Jonathan; Sandahl, Thomas Damgaard; Glavind, Emilie; Vilstrup, Hendrik; Caraceni, Paolo; Bertucci, Carlo.
Afiliación
  • Naldi M; Department of Pharmacy and Biotechnology, Via Sand Donato 15, 40127, University of Bologna, Italy; Center for Applied Biomedical Research (C.R.B.A.), S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy.
  • Baldassarre M; Center for Applied Biomedical Research (C.R.B.A.), S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy; Department of Medical and Surgical Sciences, Via Massarenti 9, 40138, University of Bologna, Italy.
  • Domenicali M; Center for Applied Biomedical Research (C.R.B.A.), S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy; Department of Medical and Surgical Sciences, Via Massarenti 9, 40138, University of Bologna, Italy.
  • Giannone FA; Center for Applied Biomedical Research (C.R.B.A.), S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy; Department of Medical and Surgical Sciences, Via Massarenti 9, 40138, University of Bologna, Italy.
  • Bossi M; Department of Medical and Surgical Sciences, Via Massarenti 9, 40138, University of Bologna, Italy.
  • Montomoli J; Department of Hepatology and Gastroenterology, Norrebrogade 44, DK-8000, Aarhus University Hospital, Denmark.
  • Sandahl TD; Department of Hepatology and Gastroenterology, Norrebrogade 44, DK-8000, Aarhus University Hospital, Denmark.
  • Glavind E; Department of Hepatology and Gastroenterology, Norrebrogade 44, DK-8000, Aarhus University Hospital, Denmark.
  • Vilstrup H; Department of Hepatology and Gastroenterology, Norrebrogade 44, DK-8000, Aarhus University Hospital, Denmark.
  • Caraceni P; Center for Applied Biomedical Research (C.R.B.A.), S. Orsola-Malpighi Hospital, Via Massarenti 9, 40138 Bologna, Italy; Department of Medical and Surgical Sciences, Via Massarenti 9, 40138, University of Bologna, Italy.
  • Bertucci C; Department of Pharmacy and Biotechnology, Via Sand Donato 15, 40127, University of Bologna, Italy. Electronic address: carlo.bertucci@unibo.it.
J Pharm Biomed Anal ; 122: 141-7, 2016 Apr 15.
Article en En | MEDLINE | ID: mdl-26852162
Human serum albumin (HSA) is the most abundant plasma protein, endowed with several biological properties unrelated to its oncotic power, such as antioxidant and free-radicals scavenging activities, binding and transport of many endogenous and exogenous substances, and regulation of endothelial function and inflammatory response. These non-oncotic activities are closely connected to the peculiarly dynamic structure of the albumin molecule. HSA undergoes spontaneous structural modifications, mainly by reaction with oxidants and saccharides; however, patients with cirrhosis show extensive post-transcriptional changes at several molecular sites of HSA, the degree of which parallels the severity of the disease. The present work reports the development and application of an innovative LC-MS analytical method for a rapid and reproducible determination of the relative abundance of HSA isoforms in plasma samples from alcoholic hepatitis (AH) patients. A condition of severe oxidative stress, similar to that observed in AH patients, is associated with profound changes in circulating HSA microheterogeneity. More interestingly, the high resolution provided by the analytical platform allowed the monitoring of novel oxidative products of HSA never reported before.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasma / Espectrometría de Masas / Albúmina Sérica / Hepatitis Alcohólica Tipo de estudio: Observational_studies / Risk_factors_studies Límite: Adult / Aged / Humans / Middle aged Idioma: En Revista: J Pharm Biomed Anal Año: 2016 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Plasma / Espectrometría de Masas / Albúmina Sérica / Hepatitis Alcohólica Tipo de estudio: Observational_studies / Risk_factors_studies Límite: Adult / Aged / Humans / Middle aged Idioma: En Revista: J Pharm Biomed Anal Año: 2016 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Reino Unido