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Surgical and Oncological Factors Affecting the Successful Engraftment of Patient-derived Xenografts in Pancreatic Ductal Adenocarcinoma.
Jun, Eunsung; Jung, Jaeyoon; Jeong, Seong-Yun; Choi, Eun Kyung; Kim, Moon Bo; Lee, Ji Sun; Hong, Seung-Mo; Seol, Hyang Sook; Hwang, Changmo; Hoffman, Robert M; Shim, In Kyong; Chang, Suhwan; Kim, Song Cheol.
Afiliación
  • Jun E; Departments of Surgery, University of Ulsan College of Medicine and Asan Medical Center, Songpa-gu, Seoul, Republic of Korea.
  • Jung J; Division of Biomedical Sciences, Department of Physiology, University of Ulsan College of Medicine and Asan Medical Center, Songpa-gu, Seoul, Republic of Korea.
  • Jeong SY; Center for Advancing Cancer Therapeutics, University of Ulsan College of Medicine and Asan Medical Center, Songpa-gu, Seoul, Republic of Korea Asan Institute for Life Sciences, University of Ulsan College of Medicine and Asan Medical Center, Songpa-gu, Seoul, Republic of Korea.
  • Choi EK; Center for Advancing Cancer Therapeutics, University of Ulsan College of Medicine and Asan Medical Center, Songpa-gu, Seoul, Republic of Korea.
  • Kim MB; Metabio, Inc. Gangdong-gu, Seoul, Republic of Korea.
  • Lee JS; Metabio, Inc. Gangdong-gu, Seoul, Republic of Korea.
  • Hong SM; Department of Pathology, University of Ulsan College of Medicine, Songpa-gu, Seoul, Republic of Korea.
  • Seol HS; Division of Biomedical Sciences, Department of Physiology, University of Ulsan College of Medicine and Asan Medical Center, Songpa-gu, Seoul, Republic of Korea.
  • Hwang C; Biomedical Engineering Research Center, Asan Institute for Life Sciences, University of Ulsan College of Medicine and Asan Medical Center, Songpa-gu, Seoul, Republic of Korea.
  • Hoffman RM; Department of Surgery, University of California, San Diego, CA, U.S.A. AntiCancer, Inc., San Diego, CA, U.S.A.
  • Shim IK; Asan Institute for Life Sciences, University of Ulsan College of Medicine and Asan Medical Center, Songpa-gu, Seoul, Republic of Korea.
  • Chang S; Division of Biomedical Sciences, Department of Physiology, University of Ulsan College of Medicine and Asan Medical Center, Songpa-gu, Seoul, Republic of Korea drksc@amc.seoul.kr suhwan.chang@amc.seoul.kr.
  • Kim SC; Departments of Surgery, University of Ulsan College of Medicine and Asan Medical Center, Songpa-gu, Seoul, Republic of Korea Asan Institute for Life Sciences, University of Ulsan College of Medicine and Asan Medical Center, Songpa-gu, Seoul, Republic of Korea drksc@amc.seoul.kr suhwan.chang@amc.seou
Anticancer Res ; 36(2): 517-21, 2016 Feb.
Article en En | MEDLINE | ID: mdl-26851005
BACKGROUND: To effectively use pancreatic cancer patient-derived xenograft (PDX) models in translational research, successful PDX engraftment of surgical specimens in immune-deficient mice is needed. MATERIALS AND METHODS: A total of 102 patients underwent pancreatic cancer resection using various procedures. Tumor tissue from all patents was implanted subcutaneously into mice. Tumor engraftment and growth in mice were determined. Engraftment was tested for correlation with operation type, time, tumor size, and oncogene expression using immunohistoculture. RESULTS: Multivariate analysis showed that a tumor size of more than 3.5 cm in the patient was a significant factor related to successful PDX engraftment. In contrast, there was no correlation of engraftment with surgical procedure, time needed to remove the specimen, tumor differentiation, lymph node metastasis, and protein expression of p53, Receptor tyrosine-protein kinase erbB-2 (CERBB2), or deleted in pancreatic carcinoma locus 4 (DPC4). CONCLUSION: A minimum tumor size in the patient is an important factor for successful tumor engraftment.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Pancreáticas / Adenocarcinoma / Biomarcadores de Tumor / Carcinoma Ductal Pancreático Tipo de estudio: Observational_studies / Prognostic_studies Límite: Animals / Female / Humans / Male / Middle aged Idioma: En Revista: Anticancer Res Año: 2016 Tipo del documento: Article Pais de publicación: Grecia
Buscar en Google
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Pancreáticas / Adenocarcinoma / Biomarcadores de Tumor / Carcinoma Ductal Pancreático Tipo de estudio: Observational_studies / Prognostic_studies Límite: Animals / Female / Humans / Male / Middle aged Idioma: En Revista: Anticancer Res Año: 2016 Tipo del documento: Article Pais de publicación: Grecia