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Histone H1 Variants in Arabidopsis Are Subject to Numerous Post-Translational Modifications, Both Conserved and Previously Unknown in Histones, Suggesting Complex Functions of H1 in Plants.
Kotlinski, Maciej; Rutowicz, Kinga; Knizewski, Lukasz; Palusinski, Antoni; Oledzki, Jacek; Fogtman, Anna; Rubel, Tymon; Koblowska, Marta; Dadlez, Michal; Ginalski, Krzysztof; Jerzmanowski, Andrzej.
Afiliación
  • Kotlinski M; Laboratory of Systems Biology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
  • Rutowicz K; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
  • Knizewski L; Laboratory of Bioinformatics and Systems Biology, Centre of New Technologies, University of Warsaw, Warsaw, Poland.
  • Palusinski A; Laboratory of Systems Biology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
  • Oledzki J; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
  • Fogtman A; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
  • Rubel T; Institute of Radioelectronic and Multimedia Technology, Warsaw University of Technology, Warsaw, Poland.
  • Koblowska M; Laboratory of Systems Biology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
  • Dadlez M; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
  • Ginalski K; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
  • Jerzmanowski A; Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland.
PLoS One ; 11(1): e0147908, 2016.
Article en En | MEDLINE | ID: mdl-26820416
Linker histones (H1s) are conserved and ubiquitous structural components of eukaryotic chromatin. Multiple non-allelic variants of H1, which differ in their DNA/nucleosome binding properties, co-exist in animal and plant cells and have been implicated in the control of genetic programs during development and differentiation. Studies in mammals and Drosophila have revealed diverse post-translational modifications of H1s, most of which are of unknown function. So far, it is not known how this pattern compares with that of H1s from other major lineages of multicellular Eukaryotes. Here, we show that the two main H1variants of a model flowering plant Arabidopsis thaliana are subject to a rich and diverse array of post-translational modifications. The distribution of these modifications in the H1 molecule, especially in its globular domain (GH1), resembles that occurring in mammalian H1s, suggesting that their functional significance is likely to be conserved. While the majority of modifications detected in Arabidopsis H1s, including phosphorylation, acetylation, mono- and dimethylation, formylation, crotonylation and propionylation, have also been reported in H1s of other species, some others have not been previously identified in histones.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Histonas / Procesamiento Proteico-Postraduccional / Arabidopsis / Proteínas de Arabidopsis Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2016 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Histonas / Procesamiento Proteico-Postraduccional / Arabidopsis / Proteínas de Arabidopsis Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2016 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Estados Unidos