Your browser doesn't support javascript.
loading
Interactions between the Powdery Mildew Effector BEC1054 and Barley Proteins Identify Candidate Host Targets.
Pennington, Helen G; Gheorghe, Dana M; Damerum, Annabelle; Pliego, Clara; Spanu, Pietro D; Cramer, Rainer; Bindschedler, Laurence V.
Afiliación
  • Pennington HG; Department of Life Sciences, Imperial College London , SW7 2AZ London, United Kingdom.
  • Gheorghe DM; Department of Life Sciences, Imperial College London , SW7 2AZ London, United Kingdom.
  • Damerum A; Department of Life Sciences, Imperial College London , SW7 2AZ London, United Kingdom.
  • Pliego C; Department of Life Sciences, Imperial College London , SW7 2AZ London, United Kingdom.
  • Spanu PD; Department of Life Sciences, Imperial College London , SW7 2AZ London, United Kingdom.
  • Cramer R; Department of Chemistry, University of Reading , Reading RG6 6AD, United Kingdom.
  • Bindschedler LV; Department of Chemistry, University of Reading , Reading RG6 6AD, United Kingdom.
J Proteome Res ; 15(3): 826-39, 2016 Mar 04.
Article en En | MEDLINE | ID: mdl-26813582
There are over 500 candidate secreted effector proteins (CSEPs) or Blumeria effector candidates (BECs) specific to the barley powdery mildew pathogen Blumeria graminis f.sp. hordei. The CSEP/BEC proteins are expressed and predicted to be secreted by biotrophic feeding structures called haustoria. Eight BECs are required for the formation of functional haustoria. These include the RNase-like effector BEC1054 (synonym CSEP0064). In order to identify host proteins targeted by BEC1054, recombinant BEC1054 was expressed in E. coli, solubilized, and used in pull-down assays from barley protein extracts. Many putative interactors were identified by LC-MS/MS after subtraction of unspecific binders in negative controls. Therefore, a directed yeast-2-hybrid assay, developed to measure the effectiveness of the interactions in yeast, was used to validate putative interactors. We conclude that BEC1054 may target several host proteins, including a glutathione-S-transferase, a malate dehydrogenase, and a pathogen-related-5 protein isoform, indicating a possible role for BEC1054 in compromising well-known key players of defense and response to pathogens. In addition, BEC1054 interacts with an elongation factor 1 gamma. This study already suggests that BEC1054 plays a central role in barley powdery mildew virulence by acting at several levels.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Hordeum / Mapeo de Interacción de Proteínas / Interacciones Huésped-Patógeno Tipo de estudio: Prognostic_studies Idioma: En Revista: J Proteome Res Asunto de la revista: BIOQUIMICA Año: 2016 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Hordeum / Mapeo de Interacción de Proteínas / Interacciones Huésped-Patógeno Tipo de estudio: Prognostic_studies Idioma: En Revista: J Proteome Res Asunto de la revista: BIOQUIMICA Año: 2016 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Estados Unidos