Invasiveness of Listeria monocytogenes strains isolated from animals in Poland.
Pol J Vet Sci
; 18(4): 697-702, 2015.
Article
en En
| MEDLINE
| ID: mdl-26812809
Animals are important reservoir of Listeria monocytogenes, a pathogen causing serious infections in both humans and livestock. However, data on invasiveness of L. monocytogenes strains of animal origin is very scarce. Ability of 18 L. monocytogenes strains of animal origin to invade HT-29 cells was investigated. Plaque forming assay was used to assess invasiveness and ability of the pathogen to spread in the cell line. Almost 40% of L. monocytogenes strains were weakly invasive. It was shown that strains from serogroup 4b exhibited the highest invasiveness, whereas serogroup 1/2b consisted of strains of invasiveness below 0.0001%. Analysis of translated inlA and inlB gene sequences revealed no premature stop codons. Lineage-specific mutations in low invasive strains were identified within inlA and inlB sequences. Our results demonstrate high incidence of low invasive animal L. monocytogenes strains, which may be at least partly explained by unique point mutations in the InlA and InlB.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Listeriosis
/
Listeria monocytogenes
Límite:
Animals
/
Humans
País/Región como asunto:
Europa
Idioma:
En
Revista:
Pol J Vet Sci
Asunto de la revista:
MEDICINA VETERINARIA
Año:
2015
Tipo del documento:
Article
Pais de publicación:
Alemania