Engineered bacterial polyester hydrolases efficiently degrade polyethylene terephthalate due to relieved product inhibition.

Wei, Ren; Oeser, Thorsten; Schmidt, Juliane; Meier, René; Barth, Markus; Then, Johannes; Zimmermann, Wolfgang

Wei, Ren; Oeser, Thorsten; Schmidt, Juliane; Meier, René; Barth, Markus; Then, Johannes; Zimmermann, Wolfgang.

Afiliación

Wei R; Department of Microbiology and Bioprocess Technology, Institute of Biochemistry, Leipzig University, Johannisallee 21-23, 04103 Leipzig, Germany.
Oeser T; Department of Microbiology and Bioprocess Technology, Institute of Biochemistry, Leipzig University, Johannisallee 21-23, 04103 Leipzig, Germany.
Schmidt J; Department of Microbiology and Bioprocess Technology, Institute of Biochemistry, Leipzig University, Johannisallee 21-23, 04103 Leipzig, Germany.
Meier R; Department of Biochemistry and Bioorganic Chemistry, Institute of Biochemistry, Leipzig University, Leipzig, Germany.
Barth M; Department of Microbiology and Bioprocess Technology, Institute of Biochemistry, Leipzig University, Johannisallee 21-23, 04103 Leipzig, Germany.
Then J; Department of Microbiology and Bioprocess Technology, Institute of Biochemistry, Leipzig University, Johannisallee 21-23, 04103 Leipzig, Germany.
Zimmermann W; Department of Microbiology and Bioprocess Technology, Institute of Biochemistry, Leipzig University, Johannisallee 21-23, 04103 Leipzig, Germany. wolfgang.zimmermann@uni-leipzig.de.

Biotechnol Bioeng ; 113(8): 1658-65, 2016 08.

Article en En | MEDLINE | ID: mdl-26804057

Asunto(s)

Proteínas Bacterianas/metabolismo; Hidrolasas/metabolismo; Poliésteres/metabolismo; Tereftalatos Polietilenos/metabolismo; Proteínas Recombinantes/metabolismo; Actinobacteria/enzimología; Actinobacteria/genética; Proteínas Bacterianas/química; Proteínas Bacterianas/genética; Biocatálisis; Estabilidad de Enzimas; Escherichia coli; Hidrolasas/química; Hidrolasas/genética; Hidrólisis; Mutagénesis Sitio-Dirigida; Poliésteres/análisis; Poliésteres/química; Tereftalatos Polietilenos/análisis; Tereftalatos Polietilenos/química; Proteínas Recombinantes/química; Proteínas Recombinantes/genética

Palabras clave

biocatalysis; hydrolase; polyester; polyethylene terephthalate; product inhibition

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Poliésteres / Proteínas Bacterianas / Proteínas Recombinantes / Tereftalatos Polietilenos / Hidrolasas Idioma: En Revista: Biotechnol Bioeng Año: 2016 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos

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