Spinal cord injury effectively ameliorated by neuroprotective effects of rosmarinic acid.
Nutr Neurosci
; 20(3): 172-179, 2017 Apr.
Article
en En
| MEDLINE
| ID: mdl-26796989
OBJECTIVE: Pathophysiology of spinal cord injury (SCI) causes primary and secondary effects leading to loss of neuronal function. The aim of the present study was to investigate the role of rosmarinic acid (RA) in protection against SCI. METHODS: The experimental study was carried out in male wistar rats categorized into three groups. Group I - sham operated rats; Group II - SCI; Group III - SCI followed by RA treatment (10â
mg/kg). The spinal tissues after treatment schedule were analyzed for oxidative stress status through determination of reactive oxygen species (ROS), lipid peroxidation, protein damage (carbonyl and sulfhydryl contents), and antioxidant enzyme activities. The expression of oxidative stress factors NF-κB and Nrf-2 was determined by Western blot analysis. Further pro-inflammatory cytokines (TNF-α, IL-6, MCP-1, and IL-1ß) were measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: The results show that treatment with RA significantly enhances the antioxidant status and decrease the oxidative stress in wistar rats post-SCI. RA effectively ameliorated inflammatory mechanisms by downregulation of NF-κB and pro-inflammatory cytokines post-SCI. CONCLUSION: The study demonstrates for the first time on the role of RA in protecting the spinal cord from injury and demonstrates its neuroprotection in wistar rats.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Médula Espinal
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Traumatismos de la Médula Espinal
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Cinamatos
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Estrés Oxidativo
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Fármacos Neuroprotectores
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Modelos Animales de Enfermedad
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Depsidos
/
Neuronas Motoras
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Nutr Neurosci
Asunto de la revista:
CIENCIAS DA NUTRICAO
/
NEUROLOGIA
Año:
2017
Tipo del documento:
Article
Pais de publicación:
Reino Unido