The in vitro effect of 1 alpha,25-dihydroxyvitamin D3 on insulin production by neonatal rat islets.

d'Emden, M C; Dunlop, M; Larkins, R G; Wark, J D

d'Emden, M C; Dunlop, M; Larkins, R G; Wark, J D.

Afiliación

d'Emden MC; University of Melbourne Department of Medicine, Royal Melbourne Hospital, Victoria, Australia.

Biochem Biophys Res Commun ; 164(1): 413-8, 1989 Oct 16.

Article en En | MEDLINE | ID: mdl-2679556

RESUMEN

The in vitro effect of 1 alpha,25-dihydroxyvitamin D3 on the function of beta cells of the endocrine pancreas was investigated. Neonatal islets maintained in serum-free medium, or medium supplemented with 0.5% fetal bovine serum achieved a 2.5-fold increase in medium insulin levels in response to 10(8) M 1 alpha,25-dihydroxyvitamin D3 (P less than 0.001). The effect of 1,25-dihydroxyvitamin D3 required at least 96 h treatment to become evident and was similar at medium glucose concentrations of 10 and 20 mM. Cell-associated insulin was increased in 1 alpha,25-dihydroxyvitamin D3-treated cultures maintained in 0.5% serum. These data suggest that 1 alpha,25-dihydroxyvitamin D3 may have a direct effect in the beta cell.

Asunto(s)

Calcitriol/farmacología; Insulina/biosíntesis; Islotes Pancreáticos/efectos de los fármacos; Animales; Animales Recién Nacidos; Técnicas In Vitro; Islotes Pancreáticos/metabolismo; Ratas; Ratas Endogámicas

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Calcitriol / Islotes Pancreáticos / Insulina Límite: Animals Idioma: En Revista: Biochem Biophys Res Commun Año: 1989 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos

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