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Same-day sputum smear microscopy for the diagnosis of pulmonary tuberculosis: direct vs. concentrated smear.
Chandra, T J; Selvaraj, R; Sharma, Y V.
Afiliación
  • Chandra TJ; Department of Microbiology, GSL Medical College, Rajahmundry, India.
  • Selvaraj R; Centre for Laboratory Animal Technology and Research, Sathyabama University, Chennai, India.
  • Sharma YV; Department of Pathology, GSL Medical College, Rajahmundry, India.
Int J Tuberc Lung Dis ; 20(2): 247-51, 2016 Feb.
Article en En | MEDLINE | ID: mdl-26792479
OBJECTIVE: To evaluate the efficacy of sputum microscopy by the concentration method using spot and morning and same-day smears for the diagnosis of pulmonary tuberculosis. METHODS: Three sputum specimens (spot, second spot and morning) were collected from study participants. Three smears were prepared from the direct sample and three using the N-acetyl-l-cysteine-sodium hydroxide concentration method. The smears were stained using Ziehl-Neelsen (ZN) staining, modified ZN (MZN) staining and fluorescent staining (FS). Smear results were pooled and compared in two categories: standard spot and morning, and same-day smears. The χ(2) test was used to evaluate the statistical difference in smear-positive cases. RESULTS: Among 3186 participants included in the study, smear positivity was respectively 9.6%, 9.8% and 10.8% for ZN, MZN and FS smears using spot and morning smears. Using same-day smears, smear positivity was respectively 9.5%, 9.8% and 10.6% for ZN, MZN and FS smears. Smear positivity increased to 16% with the concentration method. CONCLUSION: Nil dropouts and patient convenience are the added advantages of using same-day smears, and the concentration method improved smear positivity. Given these advantages, using same-day smears with the concentration method should be considered by the World Health Organization and India's Revised National Tuberculosis Control Programme.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Manejo de Especímenes / Tuberculosis Pulmonar / Técnicas Bacteriológicas / Microscopía / Mycobacterium tuberculosis Tipo de estudio: Diagnostic_studies / Evaluation_studies / Prognostic_studies Límite: Adult / Female / Humans / Male Idioma: En Revista: Int J Tuberc Lung Dis Año: 2016 Tipo del documento: Article País de afiliación: India Pais de publicación: Francia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Manejo de Especímenes / Tuberculosis Pulmonar / Técnicas Bacteriológicas / Microscopía / Mycobacterium tuberculosis Tipo de estudio: Diagnostic_studies / Evaluation_studies / Prognostic_studies Límite: Adult / Female / Humans / Male Idioma: En Revista: Int J Tuberc Lung Dis Año: 2016 Tipo del documento: Article País de afiliación: India Pais de publicación: Francia