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Translocation of Rigid Rod-Shaped Virus through Various Solid-State Nanopores.
Wu, Hongwen; Chen, Yuhao; Zhou, Qizhao; Wang, Rongliang; Xia, Baicheng; Ma, Dejun; Luo, Kaifu; Liu, Quanjun.
Afiliación
  • Wu H; State Key Laboratory of Bioelectronics, Southeast University , Nanjing, Jiangsu 210096, China.
  • Chen Y; CAS Key Laboratory of Soft Matter Chemistry and Department of Polymer Science and Engineering, University of Science and Technology of China , Hefei, Anhui 230026, China.
  • Zhou Q; The Third Affiliated Hospital of Southern Medical University , Guangzhou, Guangdong 510000, China.
  • Wang R; State Key Laboratory of Bioelectronics, Southeast University , Nanjing, Jiangsu 210096, China.
  • Xia B; CAS Key Laboratory of Soft Matter Chemistry and Department of Polymer Science and Engineering, University of Science and Technology of China , Hefei, Anhui 230026, China.
  • Ma D; State Key Laboratory of Elemento-Organic Chemistry and Department of Chemical Biology, National Pesticide Engineering Research Center (Tianjin), Nankai University , Tianjin, 300071, China.
  • Luo K; CAS Key Laboratory of Soft Matter Chemistry and Department of Polymer Science and Engineering, University of Science and Technology of China , Hefei, Anhui 230026, China.
  • Liu Q; State Key Laboratory of Bioelectronics, Southeast University , Nanjing, Jiangsu 210096, China.
Anal Chem ; 88(4): 2502-10, 2016 Feb 16.
Article en En | MEDLINE | ID: mdl-26790522
Nanopores have been used as a high throughput tool for characterizing individual biomolecules and nanoparticles. Here, we present the translocation of rigid rod-shaped tobacco mosaic virus (TMV) through solid-state nanopores. Interestingly, due to the high rigidity of TMV, three types of events with distinctive characteristics at the capture process and a strong current fluctuation during the translocation of TMV are observed. A kinetic model is then proposed to address the dynamics of the translocation, followed by corresponding dynamics simulations. The results reveal that TMV has to rotate to fit and pass the pore when it is captured by a nanopore with an angle larger than the maximum angle that allows it to pass through. Then, we investigate the dependence of the rotation of TMV on the conductance fluctuations at the blockade stage. The results show that the rotation of TMV during the passage through the pore affects the current signal significantly. This study gives a fundamental understanding of the dynamics of rod-shaped particles translocating through the nanopore and how the current responds to it. It opens a new possible way to characterize the rigidity of analytes by nanopores.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus del Mosaico del Tabaco / Nanoporos Tipo de estudio: Prognostic_studies Idioma: En Revista: Anal Chem Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus del Mosaico del Tabaco / Nanoporos Tipo de estudio: Prognostic_studies Idioma: En Revista: Anal Chem Año: 2016 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos