Possibilities of Using Fetal Hemoglobin as a Platform for Producing Hemoglobin-Based Oxygen Carriers (HBOCs).

Ratanasopa, Khuanpiroon; Cedervall, Tommy; Bülow, Leif

Ratanasopa, Khuanpiroon; Cedervall, Tommy; Bülow, Leif.

Afiliación

Ratanasopa K; Pure and Applied Biochemistry, Department of Chemistry, Lund University, 221 00, Lund, Sweden.
Cedervall T; Biochemistry and Structural Biology, Department of Chemistry, Lund University, 221 00, Lund, Sweden.
Bülow L; Pure and Applied Biochemistry, Department of Chemistry, Lund University, 221 00, Lund, Sweden. Leif.Bulow@tbiokem.lth.se.

Adv Exp Med Biol ; 876: 445-453, 2016.

Article en En | MEDLINE | ID: mdl-26782244

RESUMEN

The expression levels of fetal hemoglobin (HbF) in bacterial recombinant systems are higher compared with normal adult hemoglobin (HbA). However, heme disorientation in globins are often observed in recombinant production processes, both for HbA and HbF, although the degree of heme oriental disorder is much lower for HbF. In addition, the heme disorientation can be converted to a normal conformation by an oxidation-reduction process. A chromatographic cleaning process involving a strong anion exchanger can be utilized to remove such unstable and nondesirable forms of Hb.

Asunto(s)

Hemoglobina Fetal/metabolismo; Hemoglobinas/metabolismo; Oxígeno/metabolismo; Dicroismo Circular; Hemoglobina Fetal/análisis; Humanos

Palabras clave

Autooxidation; Circular dichroism; E. coli; Heme; Hemoglobin purification

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxígeno / Hemoglobina Fetal / Hemoglobinas Límite: Humans Idioma: En Revista: Adv Exp Med Biol Año: 2016 Tipo del documento: Article País de afiliación: Suecia Pais de publicación: Estados Unidos

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google