Intron sequence of the taurocyamine kinase gene as a marker to investigate genetic variation of Paragonimus species in Japan and the origins of triploidy in P. westermani.

Saijuntha, Weerachai; Tantrawatpan, Chairat; Jarilla, Blanca R; Agatsuma, Takeshi; Andrews, Ross H; Petney, Trevor N

Saijuntha, Weerachai; Tantrawatpan, Chairat; Jarilla, Blanca R; Agatsuma, Takeshi; Andrews, Ross H; Petney, Trevor N.

Afiliación

Saijuntha W; Walai Rukhavej Botanical Research Institute (WRBRI), Mahasarakham University, Maha Sarakham 44150, Thailand.
Tantrawatpan C; Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathum Thani 12121, Thailand talent3003@yahoo.com.
Jarilla BR; Department of Immunology, Research Institute for Tropical Medicine (RITM), Department of Health Compound, FILINVEST Corporate City, Alabang, Muntinlupa City 1781, Philippines.
Agatsuma T; Division of Environmental Health Sciences, Kochi Medical School, Oko, Nankoku 783-8505, Japan.
Andrews RH; Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Imperial College London, Faculty of Medicine, St Mary's Campus, South Wharf Street, London W2 1NY, UK Cholangiocarcinoma Screening and Care Program (CASCAP), Faculty of Medicine, Khon Kaen University, Kh
Petney TN; Institute of Zoology 1: Ecology and Parasitology, University of Karlsruhe, Kornblumen Strasse 13, Karlsruhe, Germany Cholangiocarcinoma Screening and Care Program (CASCAP), Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.

Trans R Soc Trop Med Hyg ; 110(1): 67-73, 2016 Jan.

Article en En | MEDLINE | ID: mdl-26740364

RESUMEN

BACKGROUND: Paragonimiasis is a foodborne parasitic infection caused by lung flukes of the genus Paragonimus. Several species of Paragonimus are endemic in Japan: P. westermani (diploid and triploid) P. miyazakii, P. ohirai and P. iloktsuenensis. The taxonomic status and genetic variability of these lung flukes remains poorly understood. METHODS: The second intron of domain 1 of the taurocyamine kinase gene (TKD1int2) region was used to explore genetic variation and differentiation of diploid and triploid P. westermani, as well as P. miyazakii, P. ohirai and P. iloktsuenensis originating from Japan. RESULTS: We found high levels of intraspecific variation in P. westermani, but only low levels of variation within the other species studied. Haplotype network and phylogenetic tree analyses demonstrated the sister-group relationship of P. ohirai and P. iloktsuenensis and the phylogenetically distant relationship of P. westermani with the other species. All individuals except for triploid P. westermani were homozygous. Each triploid contained at least one allele similar to that seen in most diploids from Chiba and one allele resembling that seen in diploids from Oita. One triploid contained three different sequences. CONCLUSIONS: Our findings suggested that the TKD1int2 region is a suitable marker for use in studying the genetic variation and phylogenetics of Paragonimus species, as well as providing clues to the origins of triploidy in P. westermani.

Asunto(s)

ADN de Helmintos/genética; Variación Genética/genética; Intrones/genética; Paragonimiasis/parasitología; Paragonimus/genética; Fosfotransferasas (Aceptor del Grupo Nitrogenado)/genética; Triploidía; Animales; Marcadores Genéticos; Japón; Paragonimus westermani/genética

Palabras clave

Genetic marker; Genetic variation; Heterozygosity; Intron; Paragonimus; Taurocyamine kinase

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Paragonimiasis / Paragonimus / Variación Genética / Intrones / Fosfotransferasas (Aceptor del Grupo Nitrogenado) / ADN de Helmintos / Triploidía Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Trans R Soc Trop Med Hyg Año: 2016 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Reino Unido

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