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Neurotrophin signaling in a genitofemoral nerve target organ during testicular descent in mice.
Cousinery, Mary C; Li, Ruili; Vannitamby, Amanda; Vikraman, Jaya; Southwell, Bridget R; Hutson, John M.
Afiliación
  • Cousinery MC; F Douglas Stephens, Surgical Research Group, Murdoch Childrens Research Institute, Melbourne, Australia; Department of Paediatrics, University of Melbourne, Melbourne, Australia.
  • Li R; F Douglas Stephens, Surgical Research Group, Murdoch Childrens Research Institute, Melbourne, Australia; Department of Paediatrics, University of Melbourne, Melbourne, Australia.
  • Vannitamby A; Department of Paediatrics, University of Melbourne, Melbourne, Australia.
  • Vikraman J; F Douglas Stephens, Surgical Research Group, Murdoch Childrens Research Institute, Melbourne, Australia; Department of Paediatrics, University of Melbourne, Melbourne, Australia.
  • Southwell BR; F Douglas Stephens, Surgical Research Group, Murdoch Childrens Research Institute, Melbourne, Australia; Department of Paediatrics, University of Melbourne, Melbourne, Australia.
  • Hutson JM; F Douglas Stephens, Surgical Research Group, Murdoch Childrens Research Institute, Melbourne, Australia; Department of Urology, The Royal Children's Hospital, Melbourne, Australia; Department of Paediatrics, University of Melbourne, Melbourne, Australia. Electronic address: john.hutson@rch.org.au.
J Pediatr Surg ; 51(8): 1321-6, 2016 Aug.
Article en En | MEDLINE | ID: mdl-26718832
BACKGROUND/AIM: It has been proposed that androgens control inguinoscrotal testicular descent via release of calcitonin gene-related peptide (CGRP) from a masculinised genitofemoral nerve (GFN). As there are androgen receptors in the inguinoscrotal fat pad (IFP) during the window of androgen sensitivity (E14-17 in mouse embryos), we tested the hypothesis that neurotrophins in the IFP may masculinise the sensory fibers of the GFN supplying the gubernaculum and IFP prior to gubernacular migration. METHODS: Androgen-receptor knockout (ARKO) and wild-type (WT) mouse embryos were collected at E17, with ethical approval (AEC 734). Sagittal sections of IFP, mammary area and bulbocavernosus (BC) muscle were processed for standard histology and fluorescent immunohistochemistry for ciliary neurotrophic factor (CNTF), ciliary neurotrophic factor receptor (CNTFR) and cell nuclei (DAPI). RESULTS: In the ARKO mouse CNTFR immunoreactivity (CNTFR-IR) was increased in the IFP but decreased in BC. Perinuclear staining of CNTF-IR was seen in mouse sciatic nerve but only weakly in IFP. In the mammary area, also supplied by GFN, there were no differences in IR staining. CONCLUSION: This study found CNTFR-IR in the IFP was negatively regulated by androgen, suggesting that CNTF signaling may be suppressed in GFN sensory nerves to enable CGRP expression for regulating gubernacular migration in the male, but not the female. The indirect action of androgen via the GFN required for testicular descent may be one of the sites of anomalies in the putative multifactorial cause of cryptorchidism.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Testículo / Receptores Androgénicos / Factor Neurotrófico Ciliar / Receptor de Factor Neurotrófico Ciliar / Criptorquidismo Tipo de estudio: Etiology_studies / Prognostic_studies Aspecto: Ethics Límite: Animals Idioma: En Revista: J Pediatr Surg Año: 2016 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Testículo / Receptores Androgénicos / Factor Neurotrófico Ciliar / Receptor de Factor Neurotrófico Ciliar / Criptorquidismo Tipo de estudio: Etiology_studies / Prognostic_studies Aspecto: Ethics Límite: Animals Idioma: En Revista: J Pediatr Surg Año: 2016 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos